分离和鉴定 13 个新的多态微卫星标记用于红松（松科）。
Isolation and characterization of 13 novel polymorphic microsatellite markers for Pinus koraiensis (Pinaceae).
机构信息
State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
出版信息
Am J Bot. 2012 Oct;99(10):e421-4. doi: 10.3732/ajb.1200145. Epub 2012 Oct 1.
PREMISE OF THE STUDY
Thirteen polymorphic microsatellite markers were developed to investigate the genetic diversity and population structure of Pinus koraiensis. •
METHODS AND RESULTS
Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method with three specific PCR primers for screening the positive clones, 13 loci were found to be polymorphic in 78 individuals of P. koraiensis. Across all of the P. koraiensis samples, the number of alleles per locus ranged from two to 11. •
CONCLUSIONS
These polymorphic markers will be useful for conservation genetics studies of this species and to inform the development of effective P. koraiensis conservation programs.
研究前提
开发了 13 个多态微卫星标记，用于研究红松的遗传多样性和种群结构。
方法和结果
利用基于 AFLP 的序列重复快速分离（FIASCO）方法，使用三个特定的 PCR 引物对阳性克隆进行筛选，在 78 个红松个体中发现了 13 个多态性位点。在所有红松样本中，每个位点的等位基因数范围从两个到 11 个。
结论
这些多态性标记将有助于该物种的保护遗传学研究，并为制定有效的红松保护计划提供信息。
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