大鼠背根神经节细胞中α-CGRP和β-CGRP mRNA的共表达。

Co-expression of alpha-CGRP and beta-CGRP mRNAs in the rat dorsal root ganglion cells.

作者信息

Noguchi K, Senba E, Morita Y, Sato M, Tohyama M

机构信息

Department of Anatomy II, Osaka University Medical School, Japan.

出版信息

Neurosci Lett. 1990 Jan 1;108(1-2):1-5. doi: 10.1016/0304-3940(90)90696-7.

DOI:10.1016/0304-3940(90)90696-7

PMID:2304619

Abstract

We examined the expression of alpha-calcitonin gene-related peptide (alpha-CGRP) and beta-CGRP mRNAs in the rat dorsal root ganglion (DRG) using in situ hybridization histochemistry with oligonucleotide probes. Analysis of autoradiograms showed that alpha-CGRP mRNA labeled 36.4% of all DRG neurons and beta-CGRP mRNA labeled 30.8%. Small and medium-sized neurons expressed both types of mRNA, but large neurons showed a predominance of alpha-CGRP mRNA expression. The colocalization of alpha-CGRP and beta-CGRP mRNAs was shown at a cellular level in consecutive DRG sections. Thus, we demonstrated that alpha-CGRP and beta-CGRP were distributed differently in the DRG neurons, although genes coding for these neuropeptides coexist in the small or medium-sized neurons.

摘要

我们使用寡核苷酸探针原位杂交组织化学技术检测了大鼠背根神经节（DRG）中α-降钙素基因相关肽（α-CGRP）和β-CGRP mRNA的表达。放射自显影片分析显示，α-CGRP mRNA标记了所有DRG神经元的36.4%，β-CGRP mRNA标记了30.8%。中小神经元表达两种类型的mRNA，但大神经元显示α-CGRP mRNA表达占优势。在连续的DRG切片中，在细胞水平上显示了α-CGRP和β-CGRP mRNA的共定位。因此，我们证明了α-CGRP和β-CGRP在DRG神经元中的分布不同，尽管编码这些神经肽的基因共存于中小神经元中。