胺碘酮的可见分光光度法

Visible spectrophotometric method for amiodarone.

作者信息

Bosînceanu Andreea, Popa Graţiela, Tântaru Gladiola, Popovici Iuliana

机构信息

Discipline of Pharmaceutical Technology, School of Pharmacy, University of Medicine and Pharmacy, "Grigore T. Popa", Iasi.

出版信息

Rev Med Chir Soc Med Nat Iasi. 2012 Jan-Mar;116(1):330-5.

PMID:23077917

Abstract

Amiodarone is an antiarrhythmic agent used for various types of tachyarrhythmia, both ventricular and supraventricular (atrial) arrhythmia. A spectrophotometric method for the assay of amiodarone was established. Based on the reduction of potassium ferricyanide in hydrochloric acid medium to potassium ferrocyanide forming a blue colored complex ferric ferrocyanide with Fe (III) ions. The compound was most stable in a mixture of ethylic alcohol and water (2:1, v/v) and it had an absorption maximum at 725 nm. The data were according to the Lambert-Beer Law in the concentration range of 0.5-5.0 microg/sample: correlation and coefficient R = 0.99977, R2 = 0.999541, slope of the line 0.12775, intercept 0.042077. The detection limit (DL) was 0.1032 microg/sample and the quantification limit (QL) 0.344 microg/ sample.

摘要

胺碘酮是一种抗心律失常药物，用于治疗各种类型的快速心律失常，包括室性和室上性（心房）心律失常。建立了一种分光光度法来测定胺碘酮。该方法基于在盐酸介质中，铁氰化钾被还原为亚铁氰化钾，亚铁氰化钾与Fe（III）离子形成蓝色络合物铁氰化亚铁。该化合物在乙醇和水的混合物（2:1，v/v）中最稳定，其最大吸收波长为725nm。在0.5 - 5.0μg/样品的浓度范围内，数据符合朗伯 - 比尔定律：相关系数R = 0.99977，R² = 0.999541，直线斜率为0.12775，截距为0.042077。检测限（DL）为0.1032μg/样品，定量限（QL）为0.344μg/样品。