用红球菌 PA-34 的休眠细胞将 3-氰基吡啶转化为烟酰胺的中试规模转化。
Bench scale conversion of 3-cyanopyidine to nicotinamide using resting cells of Rhodococcus rhodochrous PA-34.
机构信息
Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171 005 India.
出版信息
Indian J Microbiol. 2007 Mar;47(1):34-41. doi: 10.1007/s12088-007-0007-9. Epub 2007 Jun 14.
The nitrile hydratase (NHase, EC 3.5.5.1) activity of Rhodococcus rhodochrous PA-34 was explored for the conversion of 3-cyanopyridine to nicotinamide. The NHase activity (∼18 U/mg dry cell weight, dcw) was observed in 0.1 M phosphate buffer, pH 8.0 containing 1M 3-cyanopyridine as substrate, and 0.75 mg of resting cells (dry cell weight) per ml reaction mixture at 40°C. However, 25°C was more suitable for prolonged batch reaction at high substrate (3-cyanopyridine) concentration. In a batch reaction (1 liter), 7M 3-cyanopyridine (729 g) was completely converted to nicotinamide (855 g) in 12h at 25°C using 9.0 g resting cells (dry cell weight) of R. rhodochrous PA-34.
研究了红球菌（Rhodococcus rhodochrous）PA-34 的腈水合酶（NHase，EC 3.5.5.1）活性，以将 3-氰基吡啶转化为烟酰胺。在 0.1 M 磷酸盐缓冲液（pH 8.0）中，以 1 M 3-氰基吡啶为底物，每毫升反应混合物中含有 0.75 mg 休止细胞（干重），在 40°C 下观察到 NHase 活性（约 18 U/mg 干重）。然而，25°C 更适合在高底物（3-氰基吡啶）浓度下进行长时间分批反应。在分批反应（1 升）中，在 25°C 下，使用 9.0 g 红球菌（Rhodococcus rhodochrous）PA-34 的休止细胞（干重），12 小时内可将 7M 3-氰基吡啶（729 g）完全转化为烟酰胺（855 g）。
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