血清β-葡萄糖醛酸酶测定的改良方法：以对硝基苯葡萄糖醛酸苷和酚酞葡萄糖醛酸苷为底物在胃肠道癌中的比较研究
Modified method for determination of serum beta-glucuronidase: A comparative study using P-nitrophenyl glucuronide and phenolphthalein glucuronide as substrate in gastrointestinal tract carcinomas.
作者信息
Khanolkar M M, Sirsat A V, Walvekar S S, Bhansali M S, Desouza L J
机构信息
Department of Biochemistry, Tata Memorial Hospital, B/2, Gnyandeep CHS, 17th Road, Santacruz (West), 400 012 Mumbai, India.
出版信息
Indian J Clin Biochem. 1997 Dec;12(1):67-70. doi: 10.1007/BF02867959.
Serum beta-glucuronidase activity was estimated by our modified method using two substrates phenolphthalein glucuronide and p-nitrophenyl glucoronide in 49 healthy subjects and 94 patients with gastrointestinal tract (GIT) carcinoma. The correlation coefficient using the two substrates was found to be r=0.8383. The method of Gabor Szasz was modified wherein the incubation time was decreased from 5 hours to 2 hours and the incubation temperature was increased from 25°C to 37°C. The increase in beta-glucuronidase activity in GIT carcinoma appears to be a good biochemical marker in patients with such type of carcinomas, a finding not reported in literature to-date.The modified quantitative method used for estimation of beta-glucuronidase is reliable, accurate, simple and rapid.
采用改良方法，使用两种底物酚酞葡糖苷酸和对硝基苯基葡糖苷酸，对49名健康受试者和94名胃肠道（GIT）癌患者的血清β-葡萄糖醛酸酶活性进行了评估。发现使用这两种底物的相关系数为r = 0.8383。对加博尔·萨斯（Gabor Szasz）的方法进行了改良，将孵育时间从5小时减少至2小时，并将孵育温度从25°C提高到37°C。GIT癌患者中β-葡萄糖醛酸酶活性的增加似乎是这类癌症患者的一个良好生化标志物，这一发现迄今未见文献报道。用于评估β-葡萄糖醛酸酶的改良定量方法可靠、准确、简单且快速。
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