评价蚯蚓（赤子爱胜蚓）的 EROD 和 CYP3A4 活性作为土壤重金属污染生物标志物。
Evaluation of EROD and CYP3A4 activities in earthworm Eisenia fetida as biomarkers for soil heavy metal contamination.
机构信息
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, People's Republic of China.
出版信息
J Hazard Mater. 2012 Dec;243:146-51. doi: 10.1016/j.jhazmat.2012.10.012. Epub 2012 Oct 13.
In this study, the effects of heavy metals (Cd, Cu, Pb, Zn) on EROD and CYP3A4 activities in the earthworm Eisenia fetida were evaluated to find out their possible induction and potential as biomarkers for soil heavy metal contamination. The earthworms were exposed to increasing concentrations of Cd (0.1-8 mg L(-1)), Cu (10-200 mg L(-1)), Pb (20-400 mg L(-1)) or Zn (50-400 mg L(-1)) in filter papers for 48 h. EROD activity was significantly changed in dose-dependent manners after exposure to each of the four metals. CYP3A4 activity was significantly induced by Cd and Pb, rather by Cu and Zn. This is the first report on heavy metal-induced changes of CYP3A4 activity in earthworms. Among the four heavy metals, Cd was the most potent inducing EROD and CYP3A4. While EROD and CYP3A4 activities showed a similar trend, EROD is more sensitive than CYP3A4 activity in E. fetida as a biomarker for heavy metals pollution.
本研究旨在评估重金属（Cd、Cu、Pb、Zn）对赤子爱胜蚓（Eisenia fetida） EROD 和 CYP3A4 活性的影响，以确定它们作为土壤重金属污染生物标志物的潜在诱导作用和可能性。将蚯蚓暴露于滤纸中，浓度逐渐增加的 Cd（0.1-8mg/L）、Cu（10-200mg/L）、Pb（20-400mg/L）或 Zn（50-400mg/L）中 48 小时。四种金属暴露后，EROD 活性均呈剂量依赖性显著变化。CYP3A4 活性被 Cd 和 Pb 显著诱导，而不是 Cu 和 Zn。这是首次报道重金属诱导蚯蚓 CYP3A4 活性变化。在这四种重金属中，Cd 是最有效的 EROD 和 CYP3A4 诱导剂。虽然 EROD 和 CYP3A4 活性表现出相似的趋势，但 EROD 作为重金属污染生物标志物比 CYP3A4 活性更敏感。
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