Periodontics Department, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Arch Oral Biol. 2013 May;58(5):530-6. doi: 10.1016/j.archoralbio.2012.10.015. Epub 2012 Nov 20.
RANK/OPG/RANKL pathway plays a significant role in osteoclastogenesis, osteoclast activation, and regulation of bone resorption. The aim of this study was to investigate the association of RANKL gene polymorphisms (rs9533156 and rs2277438) with chronic periodontitis and peri-implantitis in an Iranian population.
77 patients with chronic periodontitis, 40 patients with peri-implantitis and 89 periodontally healthy patients were enrolled in this study. 5cc of blood was obtained from the cephalic vein of subjects arms and transferred into tubes containing EDTA. Genomic DNA was extracted using Miller's Salting Out technique. The DNA was transferred into 96 division plates, transported to Kbioscience Institute in United Kingdom and analyzed using the Kbioscience Competitive Allele Specific PCR (KASP) technique. Differences in the frequencies of genotypes and alleles in the disease and control groups were analyzed using Chi-square and Fisher's exact statistical tests.
Comparison of frequency of alleles in SNP rs9533156 of RANKL gene between the chronic periodontitis group with the control and peri-implantitis groups revealed statistically significant differences (P=0.024 and P=0.027, respectively). Comparison of genotype expression of SNP rs9533156 on RANKL gene between the peri-implantitis group with chronic periodontitis and control groups revealed statistically significant differences (P=0.001); the prevalence of CT genotype was significantly higher amongst the chronic periodontitis group. Regarding SNP rs2277438 of RANKL gene, comparison of prevalence of genotypes and frequency of alleles did not reveal any significant differences (P=0.641/P=0.537, respectively).
The results of this study indicate that CT genotype of rs9533156 RANKL gene polymorphism was significantly associated with peri-implantitis, and may be considered as a genetic determinant for peri-implantitis.
RANK/OPG/RANKL 通路在破骨细胞生成、破骨细胞激活和骨吸收调节中起着重要作用。本研究旨在探讨 RANKL 基因多态性（rs9533156 和 rs2277438）与伊朗人群慢性牙周炎和种植体周围炎的关系。
本研究纳入了 77 例慢性牙周炎患者、40 例种植体周围炎患者和 89 例牙周健康患者。从受试者手臂的头静脉采集 5cc 的血液，转移到含有 EDTA 的管中。采用 Miller 盐析法提取基因组 DNA。将 DNA 转移到 96 分区板中，运送到英国的 Kbioscience 研究所，并使用 Kbioscience 竞争性等位基因特异性 PCR（KASP）技术进行分析。使用卡方检验和 Fisher 精确检验统计分析疾病组和对照组基因型和等位基因频率的差异。
比较 RANKL 基因 SNP rs9533156 等位基因在慢性牙周炎组与对照组和种植体周围炎组的频率，差异有统计学意义（P=0.024 和 P=0.027）。比较 RANKL 基因 SNP rs9533156 基因型在种植体周围炎组与慢性牙周炎组和对照组之间的表达，差异有统计学意义（P=0.001）；慢性牙周炎组 CT 基因型的发生率明显更高。关于 RANKL 基因 SNP rs2277438，基因型和等位基因频率的比较均无显著差异（P=0.641/P=0.537）。
本研究结果表明，RANKL 基因 SNP rs9533156 的 CT 基因型与种植体周围炎显著相关，可能是种植体周围炎的遗传决定因素。
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