Tea Research Foundation of Kenya, P.O. Box 820, 20200 Kericho, Kenya.
Food Chem. 2013 Feb 15;136(3-4):1405-13. doi: 10.1016/j.foodchem.2012.09.066. Epub 2012 Sep 28.
Black (aerated) and green (unaerated) tea products, processed from 10 green and 18 purple leaf coloured cultivars of Kenyan origin, and two tea products, from the Japanese cultivars, Yabukita and Yutakamidori, were assayed for total polyphenols (TP) content, individual catechin profiles and in vitro antioxidant capacity (AA). In addition, the phenolic content of the tea products was determined using the Folin-Ciocalteu phenol reagent. Catechin fractions were identified using reverse phase high performance liquid chromatography (HPLC) with a binary gradient elution system. The AA% of the tea products was determined using a 2,2'-diphenyl picrylhydrazyl (DPPH) radical assay method. The results showed that TPs, catechin profiles and antioxidant activities were significantly (p≤0.05) higher in unaerated than in aerated teas. Tea products from the purple leaf coloured tea cultivars had levels of TPs, total catechin (TC) and antioxidant activities similar to those from the green leaf coloured cultivars, except for teas from the Japanese cultivars that were very low in the assayed parameters. Caffeine content was significantly (p≤0.05) lower in products from the purple leaf coloured cultivars than in those from the green leaf coloured tea cultivars. Antioxidant activity (%) was higher in tea products from the Kenyan germplasm than in those from the Japanese cultivars. Antioxidant potency of tea products was significantly (r=0.789(∗∗), p≤0.01) influenced by the total anthocyanin content of the purple leaf coloured cultivars. Cyanidin-3-O-glucoside was the anthocyanin most highly correlated with AA% (r=0.843(∗∗), p≤0.01 in unaerated tea). Total catechins in the unaerated products from the green leaf coloured tea cultivars were also significantly correlated with antioxidant capacity (r=0.818(∗∗), p≤0.01). Results from this study suggest that the antioxidant potency of teas is dependent on the predominant flavonoid compound, the type of tea cultivar and the processing method.
对来自肯尼亚的 10 个绿茶品种和 18 个紫叶品种,以及来自日本品种“Yabukita”和“Yutakamidori”的两种茶叶产品进行了总多酚(TP)含量、单体儿茶素图谱和体外抗氧化能力(AA)分析。此外,使用福林-肖卡尔特酚试剂测定茶产品的酚含量。使用反相高效液相色谱(HPLC)和二元梯度洗脱系统鉴定儿茶素级分。使用 2,2'-二苯基-1-苦基肼(DPPH)自由基测定法测定茶产品的 AA%。结果表明,未充气茶的 TPs、儿茶素图谱和抗氧化活性明显(p≤0.05)高于充气茶。紫叶茶品种的茶产品的 TPs、总儿茶素(TC)和抗氧化活性与绿叶茶品种相似,但来自日本品种的茶产品在测定参数中非常低。与绿叶茶品种相比,紫叶茶品种的产品中咖啡因含量明显(p≤0.05)较低。来自肯尼亚种质资源的茶产品的抗氧化活性(%)高于来自日本品种的茶产品。茶产品的抗氧化效力与紫叶品种的总花青素含量显著相关(r=0.789(∗∗),p≤0.01)。在未充气茶中,矢车菊素-3-O-葡萄糖苷与 AA%的相关性最高(r=0.843(∗∗),p≤0.01)。未充气绿茶品种的儿茶素总量也与抗氧化能力显著相关(r=0.818(∗∗),p≤0.01)。本研究结果表明,茶的抗氧化效力取决于主要类黄酮化合物、茶品种类型和加工方法。