School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Arch Pharm Res. 2012 Nov;35(11):1945-52. doi: 10.1007/s12272-012-1111-x. Epub 2012 Dec 4.
Two HPLC methods with diode array detection (HPLC-DAD) and electrospray ionization-mass spectrometry (HPLC-ESI/MS), respectively, were developed to investigate the differences of chemical constituents and their metabolism in gastrointestinal tract in vitro between two decoctions of crude and processed Glycyrrhizae radix. Total of eleven constituents (liquiritin apioside, liquiritin, licuraside, isoliquiritin, ononin, glycyrrhizin, liquiritigenin-7,4'-diglucoside, licorice saponin A3, 22β-acetoxylglycyrrhizic acid, licorice saponin G2, and yunganoside E2) were identified in the two decoctions, whereas lower contents of these constituents were usually found in the decoction of processed Glycyrrhizae Radix. [corrected] Furthermore, these constituents were metabolized into their respective aglycons in human intestinal bacteria juice, and the metabolism ratios were all higher in processed Glycyrrhizae Radix [corrected] decoction. No change was found in artificial gastric or intestinal juice. This study revealed that the processing can alter the contents of main constituents in crude G. radix and their metabolism in gastrointestinal tract, in which intestinal bacteria play an important role in the metabolism of licorice constituents.
建立了两种分别采用二极管阵列检测（HPLC-DAD）和电喷雾电离-质谱（HPLC-ESI/MS）的 HPLC 方法，以研究生甘草和炙甘草汤剂在胃肠道内的化学成分及其代谢的差异。在两种汤剂中鉴定出了 11 种成分（芹糖甘草苷、甘草苷、甘草素、异甘草苷、大豆素-7,4'-二葡萄糖苷、甘草酸、甘草素-7-葡萄糖醛酸苷、甘草皂苷 A3、22β-乙酰氧基甘草酸、甘草皂苷 G2 和远志糖苷 E2），而这些成分在炙甘草汤剂中的含量通常较低。[校正]此外，这些成分在人肠菌汁中代谢为各自的苷元，并且在炙甘草汤剂中的代谢比例均较高。[校正]在人工胃液或肠液中未发现变化。本研究表明，炮制可以改变生甘草中主要成分的含量及其在胃肠道内的代谢，其中肠道细菌在甘草成分的代谢中发挥重要作用。
