将两个大鼠二氢叶酸还原酶（DHFR）基因定位到第2号和第4号染色体上。

Assignment of two rat dihydrofolate (DHFR) genes to chromosomes 2 and 4.

作者信息

Hanson C, Andersson A, Islam M Q, Szpirer J, Szpirer C, Levan G

机构信息

Department of Genetics, University of Gothenburg, Sweden.

出版信息

Cytogenet Cell Genet. 1990;53(1):23-5. doi: 10.1159/000132888.

DOI:10.1159/000132888

PMID:2323224

Abstract

A panel of rat x mouse cell hybrids was used in the chromosomal mapping of the rat dihydrofolate reductase (DHFR) gene. It was determined that the probe hybridized to gene sequences on two different chromosomes (Nos. 2 and 4), possibly representing the active gene and a pseudogene. Hybridization of the DHFR probe to DNA from a methotrexate resistant rat cell line revealed that the gene on chromosome 2 was amplified, but not the gene on chromosome 4. This result was taken to suggest that the active DHFR gene is located on rat chromosome 2 and that the sequence on chromosome 4 is a pseudogene.

摘要

一组大鼠×小鼠细胞杂种被用于大鼠二氢叶酸还原酶（DHFR）基因的染色体定位。已确定该探针与两条不同染色体（第2号和第4号）上的基因序列杂交，可能分别代表活性基因和假基因。DHFR探针与甲氨蝶呤抗性大鼠细胞系的DNA杂交显示，2号染色体上的基因被扩增，但4号染色体上的基因未被扩增。这一结果表明，活性DHFR基因位于大鼠2号染色体上，而4号染色体上的序列是假基因。