断头处死和微波辐照处死的大鼠脑内氨基酸浓度

Brain amino acid concentrations in rats killed by decapitation and microwave irradiation.

作者信息

Miller J M, Jope R S, Ferraro T N, Hare T A

机构信息

Department of Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107.

出版信息

J Neurosci Methods. 1990 Mar;31(3):187-92. doi: 10.1016/0165-0270(90)90109-s.

DOI:10.1016/0165-0270(90)90109-s

PMID:2329838

Abstract

The effect of death by decapitation or focused beam microwave irradiation (FBMI) on rat brain amino acid concentrations was investigated. Twenty-nine amino acids and related compounds were measured by ion-exchange chromatography in the cerebral cortex, hippocampus, striatum and substantia nigra of male Sprague-Dawley rats killed by decapitation (n = 5) or by FBMI (n = 5). Alanine, GABA, ethanolamine and NH3 concentrations were significantly lower in all 4 brain regions of the FBMI group animals. Valine, leucine, tyrosine and phenylalanine levels were also lower in the hippocampus, striatum and substantia nigra of the FBMI group. The FBMI group showed less aspartate in the hippocampus and substantia nigra as well as less glycine in the cortex, hippocampus and striatum. In the FBMI group, the only amino acids exhibiting significantly higher levels were GSH in the striatum and substantia nigra and glutamate in the substantia nigra. These results show a significant impact of method of killing on the determination of baseline concentrations of brain amino acids.

摘要

研究了断头处死或聚焦束微波照射（FBMI）对大鼠脑氨基酸浓度的影响。通过离子交换色谱法测定了断头处死（n = 5）或FBMI处死（n = 5）的雄性Sprague-Dawley大鼠大脑皮层、海马体、纹状体和黑质中29种氨基酸及相关化合物的含量。FBMI组动物所有4个脑区的丙氨酸、γ-氨基丁酸、乙醇胺和氨浓度均显著降低。FBMI组海马体、纹状体和黑质中的缬氨酸、亮氨酸、酪氨酸和苯丙氨酸水平也较低。FBMI组海马体和黑质中的天冬氨酸较少，皮层、海马体和纹状体中的甘氨酸也较少。在FBMI组中，仅纹状体和黑质中的谷胱甘肽以及黑质中的谷氨酸水平显著升高。这些结果表明处死方法对脑氨基酸基线浓度的测定有显著影响。