Yang Wei-Ning, Li Zhi-Tong, Xie Jie-Ping, Li Sheng-Jie, Zhao Ya-Fang
Department of Chinese Medicine, Xijing Hospital, Xi'an 710032, China.
Zhen Ci Yan Jiu. 2012 Oct;37(5):369-74.
To observe the influence of electroacupuncture (EA) of "Chize" (LU 5, He-sea acupoint) and "Shangjuxu" (ST 37, lower He-sea acupoint) on mesenteric microcirculation, vasoactive intestinal peptide (VIP) levels in the lung, colon and hypothalamus tissues in rats with chronic obstructive pulmonary disease (COPD), so as to investigate its mechanism underlying improvement of lung derived intestinal disorders in clinical practice.
Thirty-two male Wistar rats were randomly divided into normal control, model, EA-Chize (LU 5, EA-LU 5) and EA-Shangjuxu (ST 37, EA-ST 37) groups, with 8 rats being in each group. COPD model was established by intratracheal infusion of Lipopolysaccharide (LPS, 1 mg/mL, 0.2 mL/rat) and forced inhaling smoke, once daily for 28 days. EA was applied to bilateral LU 5 and ST 37 for 20 min, once every other day for 12 sessions. The state of mesenteric microcirculation was observed under microscope and divided into grade 0 (stagnation of blood flow), I (slow flowing and silt-like state), II (faster flowing with slight or obvious grainy feeling) and III (fast flowing without grainy feeling). The contents of VIP in the lung, colon and hypothalamus were detected using radioimmunoassay (RIA).
Following modeling, the microvascular calibers were increased slightly in the model, EA-LU 5 and EA-ST 37 groups. Compared with the normal group, the blood flow velocity was increased significantly in model group (P < 0.05). In comparison with the model group, the blood flow velocity was reduced significantly in EA-LU 5 and EA-ST 37 groups (P < 0.01). There were no significant differences between EA-LU 5 and EA-ST 37 groups in blood flow velocity, among the four groups in VIP contents of the lung tissue (P > 0.05). The content of VIP in the colon was markedly higher in the model group than in the normal group, and that in the hypothalamus was obviously lower in the EA-LU 5 group than in the model group (all P < 0.05).
EA stimulation of "Chize" (LU 5) can notably reduce hypothalamic VIP content and slow down blood flow velocity of the mesenteric microvessels in COPD rats.
观察电针“尺泽”（肺经5穴，合穴）和“上巨虚”（胃经37穴，下合穴）对慢性阻塞性肺疾病（COPD）大鼠肠系膜微循环、肺、结肠及下丘脑组织中血管活性肠肽（VIP）水平的影响，以探讨其改善临床肺系肠道病变的机制。
将32只雄性Wistar大鼠随机分为正常对照组、模型组、电针尺泽组（肺经5穴，电针肺经5穴组）和电针上巨虚组（胃经37穴，电针胃经37穴组），每组8只。采用气管内注入脂多糖（LPS，1mg/mL，0.2mL/只）并强迫吸入烟雾的方法建立COPD模型，每日1次，共28天。将电针施加于双侧肺经5穴和胃经37穴，每次20分钟，隔日1次共12次。在显微镜下观察肠系膜微循环状态，分为0级（血流停滞）、Ⅰ级（血流缓慢呈淤泥状）、Ⅱ级（血流稍快，有轻微或明显颗粒感）和Ⅲ级（血流快无颗粒感）。采用放射免疫分析法（RIA）检测肺、结肠和下丘脑组织中VIP的含量。
造模后，模型组、电针肺经5穴组和电针胃经37穴组微血管口径略有增加。与正常组比较，模型组血流速度明显加快（P<0.05）。与模型组比较，电针肺经5穴组和电针胃经37穴组血流速度明显减慢（P<0.01）。电针肺经5穴组和电针胃经37穴组血流速度差异无统计学意义，四组肺组织VIP含量差异无统计学意义（P>0.05）。模型组结肠组织VIP含量明显高于正常组，电针肺经5穴组下丘脑组织VIP含量明显低于模型组（均P<0.05）。
电针“尺泽”（肺经5穴）可显著降低COPD大鼠下丘脑VIP含量，减慢肠系膜微血管血流速度。
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