用于离体锰增强 MRI 的活体锰增强的小鼠脑固定。
Mouse brain fixation to preserve In vivo manganese enhancement for ex vivo manganese-enhanced MRI.
机构信息
Department of Radiology, University of Nebraska Medical Center, Omaha, NE 68198, USA.
出版信息
J Magn Reson Imaging. 2013 Aug;38(2):482-7. doi: 10.1002/jmri.24005. Epub 2013 Jan 24.
PURPOSE
To develop a tissue fixation method that preserves in vivo manganese enhancement for ex vivo magnetic resonance imaging (MRI). The needs are clear, as conventional in vivo manganese-enhanced MRI (MEMRI) applied to live animals is time-limited, hence limited in spatial resolution and signal-to-noise ratio (SNR). Ex vivo applications can achieve superior spatial resolution and SNR through increased signal averaging and optimized radiofrequency coil designs. A tissue fixation method that preserves in vivo Mn(2+) enhancement postmortem is necessary for ex vivo MEMRI.
MATERIALS AND METHODS
T1 measurements and T1 -weighted MRI were performed on MnCl2 -administered mice. The mice were then euthanized and the brains were fixed using one of two brain tissue fixation methods: aldehyde solution or focused beam microwave irradiation (FBMI). MRI was then performed on the fixed brains.
RESULTS
T1 values and T1 -weighted signal contrasts were comparable between in vivo and ex vivo scans on aldehyde-fixed brains. FBMI resulted in the loss of Mn(2+) enhancement.
CONCLUSION
Aldehyde fixation, not FBMI, maintained in vivo manganese enhancement for ex vivo MEMRI.
目的
开发一种组织固定方法，以保留活体锰增强用于离体磁共振成像（MRI）。需求是明确的，因为应用于活体动物的传统活体锰增强 MRI（MEMRI）是限时的，因此空间分辨率和信噪比（SNR）有限。离体应用可以通过增加信号平均和优化射频线圈设计来实现更高的空间分辨率和 SNR。对于离体 MEMRI，需要一种在死后保留体内 Mn(2+)增强的组织固定方法。
材料和方法
对 MnCl2 给药的小鼠进行 T1 测量和 T1 加权 MRI。然后将小鼠安乐死，并使用两种脑组织固定方法之一固定大脑：醛溶液或聚焦束微波照射（FBMI）。然后对固定的大脑进行 MRI 检查。
结果
在醛固定的大脑中，体内和离体扫描的 T1 值和 T1 加权信号对比度相当。FBMI 导致 Mn(2+)增强的丧失。
结论
醛固定，而不是 FBMI，维持了离体 MEMRI 中的体内锰增强。
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