Wen-Jie Yan, Jing Yang, Tai-Lang Yin
Reproductive Center, People's Hospital, Wuhan University, Wuhan.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Nov;32(11):1554-7.
To study the effects and underlying mechanisms of Zhuyun Recipe (ZR) on the endometrial receptivity in ovarian stimulation (OS) and blastocyst implantation dysfunction (BID) mice.
Totally 200 normal female Kunming mice were randomly divided into 6 groups, i. e., the control group (Group A), the OS group (Group B), the OS + ZR group (Group C), the BID group (Group D), the BID + ZR group (Group E), and the ZR group (Group F). The pregnant mare's serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) were intraperitoneally injected to mice in Group B. Mifepristone was subcutaneously injected to mice in Group D at 9:00 am on the 4th gestation day. Corresponding medications were given to mice in Group C, E, and F at 1.5 mL/100 g by gastrogavage at 8:00 am from the first to the 4th gestation day. Eight uterus samples were collected at 9:00 pm on the 4th gestation day and fixed. The expression levels of leukemia inhibitory factor (LIF) and integrin beta3 were detected using immunohistochemical assay. The pregnant mice were sacrificed at 9:30 pm on the 8th gestation day, and their uterus were taken out. The number of blastocysts was counted.
Compared with Group A, the pregnant rate was 6.67% (1/15 cases) in Group B and 18.75% (3/16 cases) in Group D, the mean OD value of LIF was 0. 18 +/- 0.02 in Group B and 0.23 +/- 0.02 in Group D, and the mean OD value of integrin beta3 was 0.20 +/- 0.05 in Group B and 0.19 +/- 0. 02 in Group D, showing statistical difference (P < 0.01). The pregnant rate was 54.55% (12/22 cases) in Group C and 65. 22% (15/23 cases) in Group E, the mean OD value of LIF was 0.37 +/- 0. 09 in Group C and 0.39 +/- 0.02 in Group E, and the mean OD value of integrin beta3 was 0.34 +/- 0.04 in Group C and 0.38 +/- 0.08 in Group E, showing statistical difference when compared with those of Group B and Group D respectively (P < 0.05).
OS and BID had negative effects on the endometrial receptivity and hindered the blastocyst implantation. ZR could improve the uterine receptivity and elevate the pregnant rate by up-regulating the expressions of endometrial LIF and integrin beta3.
研究助孕方（ZR）对卵巢刺激（OS）和胚泡着床障碍（BID）小鼠子宫内膜容受性的影响及潜在机制。
将200只正常雌性昆明小鼠随机分为6组，即对照组（A组）、OS组（B组）、OS + ZR组（C组）、BID组（D组）、BID + ZR组（E组）和ZR组（F组）。对B组小鼠腹腔注射孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（HCG）。在妊娠第4天上午9:00对D组小鼠皮下注射米非司酮。在妊娠第1天至第4天上午8:00，按1.5 mL/100 g对C组、E组和F组小鼠进行灌胃给予相应药物。在妊娠第4天晚上9:00采集8个子宫样本并固定。采用免疫组织化学法检测白血病抑制因子（LIF）和整合素β3的表达水平。在妊娠第8天晚上9:30处死妊娠小鼠，取出子宫，计数胚泡数量。
与A组相比，B组妊娠率为6.67%（1/15例），D组为18.75%（3/16例）；B组LIF平均光密度值为0.18±0.02，D组为0.23±0.02；B组整合素β3平均光密度值为0.20±0.05，D组为0.19±0.02，差异均有统计学意义（P < 0.01）。C组妊娠率为54.55%（12/22例），E组为65.22%（15/23例）；C组LIF平均光密度值为0.37±0.09，E组为0.39±0.02；C组整合素β3平均光密度值为0.34±0.04，E组为0.38±0.08，分别与B组和D组相比差异有统计学意义（P < 0.05）。
OS和BID对子宫内膜容受性有负面影响，阻碍胚泡着床。ZR可通过上调子宫内膜LIF和整合素β3的表达来改善子宫容受性，提高妊娠率。
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