Laboratory of Plant Biotechnology, Hayyan Research Institute, University of Tabriz, Tabriz, Iran.
Pharm Biol. 2013 May;51(5):581-8. doi: 10.3109/13880209.2012.749924. Epub 2013 Feb 4.
Neuropsychiatric disorders, like anxiety and depression, are global problems for clinical researchers in neurology. Recently, some authors have shown neuroprotective and anti-inflammatory effects of Scrophularia striata Boiss (Scrophulariaceae) extract in rodents.
The purpose of the current study was to investigate the effects of S. striata extract on anxiety and depressant-like behaviors and find a possible mechanism for these impacts.
In this study, the elevated plus-maze (EPM) and forced swimming test (FST), which are useful models for selective identification of anxiolytic and antidepressant drug effects in rodents, were used. We investigated the effects of S. striata ethanol extract at different doses (20, 50, 100, 160 and 220 mg/kg) on anxiety and depression behaviors in the EPM and FST, and then we assessed the role of γ-aminobutyric acid (GABA)A receptor in modulation of the effects of S. striata extract in the brain.
Our results showed that effective doses of S. striata (100 and 160 mg/kg) increased the percentages of open arm time and entries in the EPM and decreased immobility time in the FST in comparison with control group, indicating anxiolytic and antidepressant effects, respectively. Moreover, intracerebroventricular administration of GABAA receptor agonist (muscimol; 1 µg/rat) enhanced the impact of S. striata, and GABAA receptor antagonist (bicuculline; 1 µg/rat) blocked these effects in rats, indicating that significant interactions existed between S. striata and the GABAergic system in the brain.
Findings of this study suggest that anxiolytic and antidepressant effects of S. striata may be modulated via the GABAergic system.
神经精神疾病，如焦虑和抑郁，是神经病学临床研究人员面临的全球性问题。最近，一些作者已经证明了贯叶连翘（玄参科）提取物对啮齿动物具有神经保护和抗炎作用。
本研究旨在探讨贯叶连翘提取物对焦虑和抑郁样行为的影响，并寻找其潜在作用机制。
本研究采用高架十字迷宫（EPM）和强迫游泳试验（FST），这是用于选择性鉴定抗焦虑和抗抑郁药物在啮齿动物中作用的有用模型。我们研究了贯叶连翘乙醇提取物在不同剂量（20、50、100、160 和 220mg/kg）下对 EPM 和 FST 中焦虑和抑郁行为的影响，然后评估了γ-氨基丁酸（GABA）A 受体在调节贯叶连翘提取物作用中的作用。
我们的结果表明，贯叶连翘的有效剂量（100 和 160mg/kg）可增加 EPM 中开放臂时间和进入次数的百分比，并减少 FST 中的不动时间，分别表明具有抗焦虑和抗抑郁作用。此外，脑室内给予 GABA A 受体激动剂（muscimol；1µg/大鼠）增强了贯叶连翘的作用，而 GABA A 受体拮抗剂（bicuculline；1µg/大鼠）阻断了大鼠的这些作用，表明贯叶连翘与大脑中的 GABA 能系统之间存在显著相互作用。
本研究结果表明，贯叶连翘的抗焦虑和抗抑郁作用可能通过 GABA 能系统进行调节。
Zotero 插件