Department of Radiology, Section of Pediatric Radiology, University of Michigan Health System, C.S. Mott Children's Hospital, 1540 E Hospital Dr, Ann Arbor, MI 48109-4252, USA.
Radiology. 2013 Jun;267(3):757-66. doi: 10.1148/radiol.13121775. Epub 2013 Feb 11.
To determine if acoustic radiation force impulse elastography-derived bowel wall shear wave velocity (SWV) allows distinction of acutely inflamed from fibrotic intestine in a Crohn disease animal model.
University Committee on the Use and Care of Animals approval was obtained. An acute inflammation Crohn disease model was produced by treating eight Lewis rats with a single administration of trinitrobenzenesulfonic acid (TNBS) enema, with imaging performed 2 days later in the surviving six rats. Colonic fibrosis in an additional eight Lewis rats was achieved by administering repeated TNBS enemas during 4 weeks, with imaging performed in the surviving seven rats 7 days later to allow acute inflammation resolution. Nine transcutaneous bowel wall SWV measurements were obtained from the colon in all rats without and with applied strain. Mean SWVs without and with applied strain were compared between animal cohorts by using the Student t test, and receiver operating characteristic (ROC) curves were created to assess diagnostic performance.
Mean bowel wall SWVs were significantly higher for fibrotic versus acute inflammation cohort of rats at 0% (3.4 ± 1.1 vs 2.3 ± 0.5 m/sec; P = .047) and 30% (6.3 ± 2.2 vs 3.6 ± 0.9 m/sec; P = .02) applied strain. Both acute inflammation and fibrotic cohort of rats demonstrated linear increases in mean SWV with increasing applied strain, with significantly different mean slopes (P = .02) and y-intercepts (P = .02). The area under the ROC curve of the SWV ratio (mean SWV/applied strain) for differentiating histopathologically confirmed fibrotic from inflamed bowel was 0.971.
Bowel wall SWV helps distinguish acutely inflamed from fibrotic intestine in a Crohn disease animal model.
确定声辐射力脉冲弹性成像衍生的肠壁剪切波速度（SWV）是否可区分克罗恩病动物模型中的急性炎症和纤维化肠。
获得大学动物使用和护理委员会的批准。通过单次给予三硝基苯磺酸（TNBS）灌肠来制备急性炎症性克罗恩病模型，在幸存的六只大鼠中于 2 天后进行成像。通过在 4 周内重复给予 TNBS 灌肠来制备另外八只Lewis 大鼠的结肠纤维化，在幸存的七只大鼠中于 7 天后进行成像以允许急性炎症消退。在所有大鼠中无应变和有应变情况下，从结肠获得 9 个经皮肠壁 SWV 测量值。使用学生 t 检验比较动物队列之间无应变和有应变时的平均 SWV，并创建接受者操作特征（ROC）曲线以评估诊断性能。
纤维化与急性炎症大鼠队列的平均肠壁 SWV 分别在 0%（3.4±1.1 与 2.3±0.5 m/sec；P=.047）和 30%（6.3±2.2 与 3.6±0.9 m/sec；P=.02）应变时显著更高。急性炎症和纤维化大鼠队列的平均 SWV 均随施加应变线性增加，斜率（P=.02）和 y 截距（P=.02）有显著差异。SWV 比值（平均 SWV/应变）区分组织病理学证实的纤维化与炎症肠的 ROC 曲线下面积为 0.971。
肠壁 SWV 有助于区分克罗恩病动物模型中的急性炎症和纤维化肠。
