Nagahama Institute of Bio-Science and Technology, 1266 Tamura-Cho, Nagahama, Shiga 526-0829, Japan.
J Nat Prod. 2013 Apr 26;76(4):554-8. doi: 10.1021/np300713c. Epub 2013 Feb 19.
Using a TLC autographic assay for radical-scavenging activity with the ABTS radical, the presence of lipophilic antioxidants in the larvae of the wild bruchid seed beetle Bruchidius dorsalis was detected. Assay-guided fractionation of the CHCl3-soluble fraction of the larvae resulted in the isolation of new glycerolipids, designated dorsamin-A763, -A737, -A765, -A739, and -A767, comprising 1,2-diacyl-sn-glycero-3-dehydrophenylalanine ester structural units. The ABTS radical scavenging activity of the dorsamin-A's was comparable with or stronger than that of Trolox.
采用 TLC 自显影法测定 ABTS 自由基清除活性,检测到野生豆象幼虫中存在亲脂性抗氧化剂。对幼虫的 CHCl3 可溶部分进行基于测定的分步分离,得到了新的甘油磷脂,命名为 dorsamin-A763、-A737、-A765、-A739 和 -A767,它们包含 1,2-二酰基-sn-甘油-3-脱水苯丙氨酸酯结构单元。dorsamin-A 的 ABTS 自由基清除活性与 Trolox 相当或更强。