拟南芥胆碱酯酶基因的玉米同源基因编码一个 GDSL 脂肪酶。
The Arabidopsis thaliana ortholog of a purported maize cholinesterase gene encodes a GDSL-lipase.
机构信息
School of Life Sciences and The Biodesign Institute, Arizona State University, P.O. Box 874501, Tempe, AZ 85287-4501, USA.
出版信息
Plant Mol Biol. 2013 Apr;81(6):565-76. doi: 10.1007/s11103-013-0021-8. Epub 2013 Feb 22.
Abstract
Acetylcholinesterase is an enzyme that is intimately associated with regulation of synaptic transmission in the cholinergic nervous system and in neuromuscular junctions of animals. However the presence of cholinesterase activity has been described also in non-metazoan organisms such as slime molds, fungi and plants. More recently, a gene purportedly encoding for acetylcholinesterase was cloned from maize. We have cloned the Arabidopsis thaliana homolog of the Zea mays gene, At3g26430, and studied its biochemical properties. Our results indicate that the protein encoded by the gene exhibited lipase activity with preference to long chain substrates but did not hydrolyze choline esters. The At3g26430 protein belongs to the SGNH clan of serine hydrolases, and more specifically to the GDS(L) lipase family.
摘要
乙酰胆碱酯酶是一种与胆碱能神经系统中的突触传递以及动物的神经肌肉接头的调节密切相关的酶。然而，胆碱酯酶活性也存在于非后生动物如粘菌、真菌和植物中。最近，据称从玉米中克隆了乙酰胆碱酯酶基因。我们已经从拟南芥中克隆了玉米基因的同源物 At3g26430，并研究了它的生化特性。我们的结果表明，该基因编码的蛋白质表现出脂肪酶活性，优先作用于长链底物，但不水解胆碱酯。At3g26430 蛋白属于 SGNH 族丝氨酸水解酶，更具体地说，属于 GDS(L)脂肪酶家族。
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