UQCRB 的功能抑制可抑制斑马鱼的血管生成。
Functional inhibition of UQCRB suppresses angiogenesis in zebrafish.
机构信息
Chemical Genomics National Research Laboratory, Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science & Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
出版信息
Biochem Biophys Res Commun. 2013 Apr 19;433(4):396-400. doi: 10.1016/j.bbrc.2013.02.082. Epub 2013 Feb 28.
Abstract
As a subunit of mitochondrial complex III, UQCRB plays an important role in complex III stability, electron transport, and cellular oxygen sensing. Herein, we report UQCRB function regarding angiogenesis in vivo with the zebrafish (Danio rerio). UQCRB knockdown inhibited angiogenesis in zebrafish leading to the suppression of VEGF expression. Moreover, the UQCRB-targeting small molecule terpestacin also inhibited angiogenesis and VEGF levels in zebrafish, supporting the role of UQCRB in angiogenesis. Collectively, UQCRB loss of function by either genetic and pharmacological means inhibited angiogenesis, indicating that UQCRB plays a key role in this process and can be a prognostic marker of angiogenesis- and mitochondria-related diseases.
摘要
作为线粒体复合物 III 的亚单位，UQCRB 在复合物 III 的稳定性、电子传递和细胞氧感应中发挥重要作用。在这里，我们报告了 UQCRB 在体内血管生成方面的功能，以斑马鱼（Danio rerio）为模型。UQCRB 敲低抑制了斑马鱼的血管生成，导致 VEGF 表达受到抑制。此外，靶向 UQCRB 的小分子 terpestacin 也抑制了斑马鱼的血管生成和 VEGF 水平，支持 UQCRB 在血管生成中的作用。综上所述，通过遗传和药理学手段抑制 UQCRB 的功能会抑制血管生成，表明 UQCRB 在这一过程中发挥着关键作用，并且可以作为血管生成和线粒体相关疾病的预后标志物。
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