Caffara M, Bruni G, Paoletti C, Gustinelli A, Fioravanti M L
Department of Veterinary Medical Sciences,Alma Mater Studiorum University of Bologna,Italy.
Department of Evolutionary Biology 'Leo Pardi',University of Florence,Italy.
J Helminthol. 2014 Sep;88(3):278-85. doi: 10.1017/S0022149X13000151. Epub 2013 Mar 18.
Adults of Clinostomum spp. are digenetic trematodes found in fish-eating birds, reptiles and occasionally mammals, including humans. Freshwater snails serve as first intermediate hosts and many fish species and amphibians as second intermediate hosts. To date, amphibian hosts of Clinostomum metacercariae include members of urodele and anuran families in North America, but no data are available on infections of European amphibians, including newts. In this study, we characterize infections of Clinostomum complanatum metacercariae in four smooth (Lissotriton vulgaris) and 18 Italian crested newts (Triturus carnifex) from an artificial pond located in a protected area in Tuscany, Italy. Parasites were surgically removed from the infected newts and identified both morphologically and using sequences of a mitochondrial gene, cytochrome c oxidase I, and the ribosomal markers, internal transcribed spacers. This is the first record of C. complanatum in European newts and, more generally, in amphibians in Europe.
斜睾吸虫属的成虫是复殖吸虫，见于食鱼鸟类、爬行动物，偶尔也见于包括人类在内的哺乳动物。淡水螺作为第一中间宿主，许多鱼类和两栖动物作为第二中间宿主。迄今为止，北美隐孔吸虫尾蚴的两栖类宿主包括有尾目和无尾目家族的成员，但尚无关于欧洲两栖动物（包括蝾螈）感染情况的数据。在本研究中，我们对来自意大利托斯卡纳一个保护区人工池塘的4只光滑蝾螈（Lissotriton vulgaris）和18只意大利有冠蝾螈（Triturus carnifex）体内的扁形隐孔吸虫尾蚴感染情况进行了特征描述。通过手术从受感染的蝾螈体内取出寄生虫，并通过形态学以及线粒体基因细胞色素c氧化酶I序列和核糖体标记内部转录间隔区进行鉴定。这是欧洲蝾螈中，更普遍地说是欧洲两栖动物中首次记录到扁形隐孔吸虫。
