锁核酸/DNA杂交寡聚物内化进入大肠杆菌。
Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli.
作者信息
Traglia German M, Sala Carol Davies, Fuxman Bass Juan I, Soler-Bistué Alfonso J C, Zorreguieta Angeles, Ramírez María Soledad, Tolmasky Marcelo E
机构信息
Institute of Microbiology and Medical Parasitology, National Scientific and Technical Research Council (CONICET), University of Buenos Aires , Buenos Aires, Argentina .
出版信息
Biores Open Access. 2012 Oct;1(5):260-3. doi: 10.1089/biores.2012.0257.
Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a stationary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.
细胞内递送对于反义技术的实际应用至关重要。将杂交锁定核酸（LNA）/DNA CAAGTACTGTTCCACCA（LNA残基加下划线）与Alexa Fluor 488偶联进行标记（fLNA/DNA），并测试其穿透大肠杆菌细胞并到达细胞质的能力。流式细胞术分析表明，fLNA/DNA与稳定期培养的14%的细胞相关联，而与标记的等序列寡脱氧核苷酸的关联可忽略不计。激光扫描共聚焦显微镜证实fLNA/DNA位于细胞质内。
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