钆特醇：一种新的、临床适用的磁共振成像示踪关节炎干细胞的标签。
Ferumoxytol: a new, clinically applicable label for stem-cell tracking in arthritic joints with MRI.
机构信息
Department of Radiology & Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
出版信息
Nanomedicine (Lond). 2013 Dec;8(12):1969-83. doi: 10.2217/nnm.12.198. Epub 2013 Mar 27.
AIM
To develop a clinically applicable MRI technique for tracking stem cells in matrix-associated stem-cell implants, using the US FDA-approved iron supplement ferumoxytol.
MATERIALS & METHODS: Ferumoxytol-labeling of adipose-derived stem cells (ADSCs) was optimized in vitro. A total of 11 rats with osteochondral defects of both femurs were implanted with ferumoxytol- or ferumoxides-labeled or unlabeled ADSCs, and underwent MRI up to 4 weeks post matrix-associated stem-cell implant. The signal-to-noise ratio of different matrix-associated stem-cell implant was compared with t-tests and correlated with histopathology.
RESULTS
An incubation concentration of 500 µg iron/ml ferumoxytol and 10 µg/ml protamine sulfate led to significant cellular iron uptake, T2 signal effects and unimpaired ADSC viability. In vivo, ferumoxytol- and ferumoxides-labeled ADSCs demonstrated significantly lower signal-to-noise ratio values compared with unlabeled controls (p < 0.01). Histopathology confirmed engraftment of labeled ADSCs, with slow dilution of the iron label over time.
CONCLUSION
Ferumoxytol can be used for in vivo tracking of stem cells with MRI.
目的
开发一种临床适用的 MRI 技术，用于跟踪基质相关干细胞植入物中的干细胞，使用美国食品和药物管理局批准的铁补充剂 ferumoxytol。
材料与方法
在体外优化脂肪来源干细胞（ADSCs）的 ferumoxytol 标记。共有 11 只双侧股骨有骨软骨缺损的大鼠分别植入 ferumoxytol 或 ferumoxides 标记或未标记的 ADSCs，并在基质相关干细胞植入后 4 周内进行 MRI 检查。使用 t 检验比较不同基质相关干细胞植入物的信噪比，并与组织病理学相关联。
结果
孵育浓度为 500μg 铁/ml ferumoxytol 和 10μg/ml 鱼精蛋白硫酸盐可导致细胞内铁摄取、T2 信号效应和 ADSC 活力不受影响。在体内，ferumoxytol 和 ferumoxides 标记的 ADSCs 的信噪比值明显低于未标记的对照组（p<0.01）。组织病理学证实了标记的 ADSCs 的植入，随着时间的推移铁标记物的缓慢稀释。
结论
Ferumoxytol 可用于 MRI 对干细胞进行体内跟踪。
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