结肠癌血清 microRNA 谱的鉴定。
Identification of serum microRNA profiles in colon cancer.
机构信息
Department of Oncology, St Olavs Hospital, Trondheim University Hospital, Olav Kyrresgt 17, Trondheim 7006, Norway.
出版信息
Br J Cancer. 2013 Apr 30;108(8):1712-9. doi: 10.1038/bjc.2013.121. Epub 2013 Apr 4.
BACKGROUND
microRNAs (miRNAs) exist in blood in an apparently stable form. We have explored whether serum miRNAs can be used as non-invasive early biomarkers of colon cancer.
METHODS
Serum samples from 30 patients with colon cancer stage IV and 10 healthy controls were examined for the expression of 375 cancer-relevant miRNAs. Based on the miRNA profile in this study, 34 selected miRNAs were measured in serum from 40 patients with stage I-II colon cancer and from 10 additional controls.
RESULTS
Twenty miRNAs were differentially expressed in serum from stage IV patients compared with controls (P<0.01). Unsupervised clustering revealed four subgroups; one corresponding mostly to the control group and the three others to the patient groups. Of the 34 miRNAs measured in the follow-up study of stage I-II patients, 21 showed concordant expression between stage IV and stage I-II patient. Based on the profiles of these 21 miRNAs, a supervised linear regression analysis (Partial Least Squares Regression) was performed. Using this model we correctly assigned stage I-II colon cancer patients based on miRNA profiles of stage IV patients.
CONCLUSION
Serum miRNA expression profiling may be utilised in early detection of colon cancer.
背景
microRNAs（miRNAs）以明显稳定的形式存在于血液中。我们探索了血清 miRNAs 是否可作为结肠癌的非侵入性早期生物标志物。
方法
检测了 30 例 IV 期结肠癌患者和 10 例健康对照者的血清样本，以检测 375 种与癌症相关的 miRNAs 的表达情况。根据本研究中的 miRNA 图谱，在 40 例 I-II 期结肠癌患者和另外 10 例对照者的血清中测量了 34 种选定的 miRNAs。
结果
与对照组相比，IV 期患者血清中有 20 种 miRNAs 表达差异显著（P<0.01）。无监督聚类显示有四个亚群；一个主要对应对照组，另外三个对应患者组。在 I-II 期患者的随访研究中测量的 34 种 miRNAs 中，有 21 种在 IV 期和 I-II 期患者之间的表达一致。基于这 21 种 miRNAs 的图谱，进行了监督线性回归分析（偏最小二乘回归）。使用该模型，我们根据 IV 期患者的 miRNA 图谱正确分配了 I-II 期结肠癌患者。
结论
血清 miRNA 表达谱分析可用于结肠癌的早期检测。
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