牛乳腺炎分离株大肠杆菌血清型 O174 O-抗原多糖的结构研究。

Structural studies of the O-antigenic polysaccharide of the bovine mastitis isolate Escherichia coli serotype O174.

机构信息

Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Borstel, Germany.

出版信息

Carbohydr Res. 2013 May 24;373:18-21. doi: 10.1016/j.carres.2013.03.004. Epub 2013 Mar 16.

DOI:10.1016/j.carres.2013.03.004

PMID:23567515

Abstract

Escherichia coli is one of the major causative agents of bovine mastitis, a disease affecting dairy herds. The lipopolysaccharide (LPS) of E. coli was plays a prominent role during infection. Here, we report on the O-antigen chemical structure of the LPS from Escherichia coli strain 2188 (serotype O174:H28) isolated from a mastitis-diseased cow. The structure of the OPS was analyzed by 1 and 2D NMR spectroscopy, and methylation analysis, which identified the branched repeating tetrasaccharide biological unit. [structure: see text].

摘要

大肠杆菌是引起奶牛乳腺炎的主要病原体之一，这种疾病会影响奶牛群。大肠杆菌的脂多糖（LPS）在感染过程中起着突出的作用。在这里，我们报告了从乳腺炎病牛中分离的大肠杆菌 2188 株（血清型 O174：H28）的 LPS 的 O-抗原化学结构。通过 1 和 2D NMR 光谱和甲基化分析对 OPS 结构进行了分析，确定了支链重复四糖生物单元。[结构：见正文]。