叶酸修饰的壳聚糖纳米颗粒的生物相容性
Biocompatibility of folate-modified chitosan nanoparticles.
作者信息
Chakraborty Subhankari Prasad, Sahu Sumanta Kumar, Pramanik Panchanan, Roy Somenath
机构信息
Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore-721 102, West Bengal, India.
出版信息
Asian Pac J Trop Biomed. 2012 Mar;2(3):215-9. doi: 10.1016/S2221-1691(12)60044-6.
OBJECTIVE
To evaluate the acute toxicity of carboxymethyl chitosan-2, 2' ethylenedioxy bis-ethylamine-folate (CMC-EDBE-FA) and as well as possible effect on microbial growth and in vitro cell cyto-toxicity.
METHODS
CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2, 2' ethylenedioxy bis-ethylamine. In vivo acute toxicity, in vitro cyto-toxicity and antimicrobial activity of CMC-EDBE-FA nanoparticle were determined.
RESULTS
Vancomycin exhibited the antibacterial activity against vancomycin sensitive Staphylococcus aureus, but CMC-EDBE-FA nanoparticle did not give any antibacterial activity as evidenced by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), disc agar diffusion (DAD) and killing kinetic assay. Further, the CMC-EDBE-FA nanoparticle showed no signs of in vivo acute toxicity up to a dose level of 1 000 mg/kg p.o., and as well as in vitro cyto-toxicity up to 250 µg/mL.
CONCLUSIONS
These findings suggest that CMC-EDBE-FA nanoparticle is expected to be safe for biomedical applications.
目的
评估羧甲基壳聚糖-2,2'-乙二氧基双乙胺-叶酸（CMC-EDBE-FA）的急性毒性以及对微生物生长和体外细胞毒性的可能影响。
方法
通过2,2'-乙二氧基双乙胺将叶酸共价连接到羧甲基壳聚糖上制备CMC-EDBE-FA。测定了CMC-EDBE-FA纳米颗粒的体内急性毒性、体外细胞毒性和抗菌活性。
结果
万古霉素对万古霉素敏感的金黄色葡萄球菌具有抗菌活性，但CMC-EDBE-FA纳米颗粒未表现出任何抗菌活性，这通过最低抑菌浓度（MIC）、最低杀菌浓度（MBC）、纸片琼脂扩散法（DAD）和杀菌动力学试验得以证明。此外，在口服剂量高达1000 mg/kg时，CMC-EDBE-FA纳米颗粒未显示出体内急性毒性迹象，在浓度高达250μg/mL时也未表现出体外细胞毒性。
结论
这些发现表明CMC-EDBE-FA纳米颗粒有望在生物医学应用中是安全的。
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