沙棘 EST 基础新型 SSR 标记的开发。
Development of EST-based new SSR markers in seabuckthorn.
机构信息
University School of Biotechnology, Guru Gobind Singh Indraprastha University, Kashmere Gate, Delhi, 110403 India.
出版信息
Physiol Mol Biol Plants. 2010 Dec;16(4):375-8. doi: 10.1007/s12298-010-0037-3. Epub 2010 Dec 9.
Abstract
EST-based SSR markers were developed by screening a collection of 1584 clustered ESTs of seabuckthorn (Hippophae rhamnoides). PCR primers were designed for the amplification of 30 microsatellite loci. Two to five allelic bands were displayed by nine primer pairs in H. rhamnoides genotypes and by eleven primer pairs in H. salicifolia genotypes. None of the thirty primer pairs detected polymorphism in H. tibetana genotypes. Considering the high polymorphism detected in the tested genotypes and their direct origin from the genic regions, these EST-SSR markers hold immense promise in seabuckthorn genome analysis, molecular breeding and population genetics.
摘要
基于 EST 的 SSR 标记是通过筛选沙棘（Hippophae rhamnoides）的 1584 个聚类 EST 库开发的。设计了 PCR 引物，用于扩增 30 个微卫星位点。在 H. rhamnoides 基因型中，9 对引物显示出 2-5 条等位基因带，在 H. salicifolia 基因型中，11 对引物显示出 2-5 条等位基因带。在 H. tibetana 基因型中，没有一对 30 对引物检测到多态性。考虑到在所测试的基因型中检测到的高多态性及其直接来源于基因区域，这些 EST-SSR 标记在沙棘基因组分析、分子育种和群体遗传学中具有巨大的应用前景。
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