雪腐镰刀菌烯醇的纯化与定量。

Purification and quantification of nivalenol.

机构信息

Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden.

出版信息

Mycotoxin Res. 1996 Sep;12(2):79-90. doi: 10.1007/BF03192266.

DOI:10.1007/BF03192266

Abstract

A mean of 2.7 g fusarenon-X (FUS-X)/L was produced in cultures ofFusarium sp Fn-2B on SSA, a synthetic medium containing sucrose and asparagine. When culturing Fn-2B on several other media, or using otherFusarium strains, much lower concentrations of FUS-X or nivalenol (NIV) were obtained. The SSA-incubations with Fn-2B in an optimal volume of 100 mL were stopped just before reaching maximal FUS-X concentration and the start of conversion to NIV. FUS-X was extracted from the 22 days old cultures and partially purified on a silica gel column. It was then hydrolysed to NIV, which was rechromatographed on silica and crystallized.The purity and the chemical and physical properties of the NIV produced were investigated. Potential sources of error when quantifying NIV were also studied.

摘要

在含有蔗糖和天冬酰胺的合成培养基 SSA 上培养镰孢菌 Fn-2B，平均产生 2.7 g/L 的呋霜灵（FUS-X）。在其他几种培养基上培养 Fn-2B，或使用其他镰孢菌菌株，得到的 FUS-X 或雪腐镰刀菌烯醇（NIV）浓度要低得多。在最佳体积 100 mL 的 SSA 孵育中，Fn-2B 的培养在达到最大 FUS-X 浓度和开始转化为 NIV 之前停止。从 22 天龄的培养物中提取 FUS-X，并在硅胶柱上进行部分纯化。然后将其水解为 NIV，在硅胶上再次进行色谱分离并结晶。研究了所产生 NIV 的纯度以及化学和物理性质。还研究了定量 NIV 时可能出现的误差源。