玉米中伏马菌素的测定：两种净化程序的评估。

Determination of fumonisins in corn: Evaluation of two purification procedures.

机构信息

Programme on Mycotoxins and Experimental Carcinogenesis, Medical Research Council, P O Box 19070, 7505, Tygerberg, South Africa.

出版信息

Mycotoxin Res. 1994 Mar;10(1):9-14. doi: 10.1007/BF03192246.

DOI:10.1007/BF03192246

PMID:23605917

Abstract

The performance of two solid phase extraction (SPE) purification procedures, used in the determination of fumonlsin B1 (FB1), B2 (FB2) and B3 (FB2) In corn, was evaluated using both thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). Fewer interferences were observed In extracts prepared using the strong anion exchange (SAX) media, in contrast to those purified on C18 media, where on occasions, visual discernment of the TLC bands was hampered by the presence of interfering compounds. Precipitate formation, resulting In the blocking of SPE cartridges was also encountered when using the C18 procedure. HPLC analyses of extracts prepared by both media indicated that they gave comparable fumonlsin recoveries from naturally contaminated corn samples. The results suggest that the C18 procedure, originally developed for the TLC analyses of FB1 in mixed feeds, may also be applied to the determination of FB2 and FB2. However, where TLC is used quantitatively for fumonlsin levels <1 μg/g, purification of sample extracts on SAX media is recommended.

摘要

两种固相萃取（SPE）净化程序的性能，用于测定玉米中的伏马菌素 B1（FB1）、B2（FB2）和 B3（FB2），使用薄层色谱（TLC）和高效液相色谱（HPLC）进行了评估。与使用 C18 介质净化相比，在使用强阴离子交换（SAX）介质制备的提取物中观察到的干扰较少，在某些情况下，由于存在干扰化合物，TLC 带的视觉辨别受到阻碍。当使用 C18 程序时，还会遇到沉淀形成，导致 SPE 小柱堵塞。两种介质制备的提取物的 HPLC 分析表明，它们从受污染的玉米样品中得到了可比的伏马菌素回收率。结果表明，最初为混合饲料中 FB1 的 TLC 分析开发的 C18 程序也可用于 FB2 和 FB2 的测定。然而，当 TLC 用于定量测定 <1μg/g 的伏马菌素水平时，建议在 SAX 介质上净化样品提取物。