乙二胺四乙酸二钾真空管品牌作为常规血液学检测中分析前变异的新来源。

Brand of dipotassium EDTA vacuum tube as a new source of pre-analytical variability in routine haematology testing.

作者信息

Lima-Oliveira G, Lippi G, Salvagno G L, Montagnana M, Poli G, Solero G P, Picheth G, Guidi G C

机构信息

Laboratory of Clinical Biochemistry, Department of Life and Reproduction Sciences, University of Verona, Italy.

出版信息

Br J Biomed Sci. 2013;70(1):6-9. doi: 10.1080/09674845.2013.11669922.

DOI:10.1080/09674845.2013.11669922

PMID:23617091

Abstract

This study assesses the use of different dry K2 (dipotassium) EDTA vacuum tubes and whether or not they might represent a bias in haematological testing. Blood was collected in three dipotassium EDTA vacuum tubes from different manufacturers: Venosafe, Vacuette and Vacutainer. Samples were analysed on an Advia 2120i analyser. Significant differences among results and biases were compared with current quality specifications. Significant differences were found for haematocrit (HCT), mean corpuscular volume (MCV), white blood cell count (WBC) and platelet distribution width (PDW) when comparing Venosafe vs. Vacuette; for MCV, WBC and PDW when comparing Venosafe vs. Vacutainer; and for HCT and MCV when comparing Vacuette vs. Vacutainer. Clinically significant variations were observed for HCT and PDW in Venosafe vs. Vacuette; PDW in Venosafe vs. Vacutainer; and HCT and MCV in Vacuette vs. Vacutainer. The use of dipotassium EDTA vacuum tubes from different manufacturers represent a clinically relevant source of variation for HCT, MCV and PDW.

摘要

本研究评估了不同的干燥K2（二钾）乙二胺四乙酸真空管的使用情况，以及它们是否可能在血液学检测中造成偏差。从不同制造商处采集了三支二钾乙二胺四乙酸真空管中的血液：Venosafe、Vacuette和Vacutainer。样本在Advia 2120i分析仪上进行分析。将结果和偏差的显著差异与当前质量规范进行比较。比较Venosafe与Vacuette时，发现血细胞比容（HCT）、平均红细胞体积（MCV）、白细胞计数（WBC）和血小板分布宽度（PDW）存在显著差异；比较Venosafe与Vacutainer时，发现MCV、WBC和PDW存在显著差异；比较Vacuette与Vacutainer时，发现HCT和MCV存在显著差异。在Venosafe与Vacuette比较中，观察到HCT和PDW有临床显著差异；在Venosafe与Vacutainer比较中，观察到PDW有临床显著差异；在Vacuette与Vacutainer比较中，观察到HCT和MCV有临床显著差异。使用不同制造商的二钾乙二胺四乙酸真空管是HCT、MCV和PDW临床上相关的变异来源。