The State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, China.
FEMS Microbiol Lett. 2013 Jul;344(2):130-7. doi: 10.1111/1574-6968.12165. Epub 2013 May 13.
RNase III is a group of dsRNA-specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in Anabaena sp. PCC7120 are highly similar to RNase III enzymes. In vitro, recombinant Alr0280 showed RNase III activity. In the same cyanobacterium, the expression of ftsH (FtsH protease) could be suppressed by overexpression of an artificial sense RNA (aaftsH) that was complementary to aftsH, an internal antisense RNA. The aaftsH interference was abolished by inactivation of alr0280, the RNase III-encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to hen1, an RNA methyltransferase gene, may also be required for the aaftsH interference. This is the first report of RNase III-dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.
RNase III 是一类 dsRNA 特异性核糖核酸酶，在 RNA 加工和代谢中发挥重要作用。鱼腥藻 PCC7120 中的 Alr0280 和 All4107 与 RNase III 酶高度相似。体外实验表明重组 Alr0280 具有 RNase III 活性。在同一种蓝藻中，ftsH（FtsH 蛋白酶）的表达可以通过过表达与 ftsH 互补的人工 sense RNA（aaftsH）来抑制，该人工 sense RNA 是 ftsH 的内部反义 RNA。alr0280（编码 RNase III 的基因）失活可消除 aaftsH 的干扰，并可通过突变体的互补恢复干扰。一种类似于 RNA 甲基转移酶基因 hen1 的蓝藻同源物可能也是 aaftsH 干扰所必需的。这是首次报道在蓝藻中存在 RNase III 依赖性 sense RNA 干扰，其潜在机制尚待阐明。
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