波兰人群（罗兹大都市区研究）中哮喘患者转化生长因子β-1表达的鉴定与关联
Identification and association of TGFβ-1 expression in patients with asthma in a Polish population - Lodz metropolitan area study.
作者信息
Panek Michał, Pietras Tadeusz, Szemraj Janusz, Fabijan Artur, Kuna Piotr
机构信息
Department of Internal Medicine, Asthma and Allergy, Medical University of Lodz 22 Kopcinskiego Str., 90-153 Lodz, Poland.
出版信息
Int J Biochem Mol Biol. 2013 Mar 31;4(1):67-74. Print 2013.
OBJECTIVE
Interaction of genotype and environment results in a specific phenotype of the clinical course of asthma. TGFβ-1 gene belongs to the important group of genes involved in the regulation of proliferation, differentiation, adhesion, and migration of a variety of cell types. TGFβ-1 is inhibitory for B and T cells, as well as IgE production. In particular, it is engaged in inflammation of the bronchi and airway remodeling in asthma, which processes are critical in the pathogenesis of the disease. The aim of this study was to evaluate the correlation between the level of expression of TGFβ-1 and the severity of asthma.
METHODS
The study included 39 participants (20 healthy subjects and 19 patients with asthma). Each sample was analysed by using real time PCR.
RESULTS
There was statistical associations between the control group and the group of patients (p = 0,00007). It was demonstrated strong correlation between healthy and patients with severe asthma according GINA guidelines (p = 0,017). It was found the strong statistical correlation between healthy and patients with severe corticosteroid dependent asthma (p = 0,013). Correlations were observed between levels of asthma severity according to the ATS guidelines and controls. The influence of the level of TGFβ-1 mRNA expression and the severity of asthma (ATS) in the FEV1 (%) parameter value was found.
CONCLUSION
It was found that an important role is played by TGFβ-1 in the pathogenesis of asthma.
目的
基因型与环境的相互作用导致哮喘临床病程出现特定表型。转化生长因子β-1（TGFβ-1）基因属于参与多种细胞类型增殖、分化、黏附和迁移调控的重要基因群组。TGFβ-1对B细胞和T细胞以及IgE产生具有抑制作用。特别是，它参与哮喘中的支气管炎症和气道重塑，这些过程在该疾病的发病机制中至关重要。本研究的目的是评估TGFβ-1表达水平与哮喘严重程度之间的相关性。
方法
该研究纳入了39名参与者（20名健康受试者和19名哮喘患者）。每个样本均采用实时荧光定量聚合酶链反应（real time PCR）进行分析。
结果
对照组与患者组之间存在统计学关联（p = 0.00007）。根据全球哮喘防治创议（GINA）指南，健康人与重度哮喘患者之间显示出强相关性（p = 0.017）。发现健康人与重度糖皮质激素依赖型哮喘患者之间存在强统计学相关性（p = 0.013）。观察到根据美国胸科学会（ATS）指南的哮喘严重程度水平与对照组之间的相关性。发现了TGFβ-1 mRNA表达水平对第一秒用力呼气容积（FEV1）（%）参数值中哮喘严重程度（ATS）的影响。
结论
发现TGFβ-1在哮喘发病机制中发挥重要作用。
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