Chen Bin, Che Tuanjie, Bai Decheng, He Xiangyi
Dept. of Prosthodontics, School of Stomatology, Lanzhou University, Lanzhou 730000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2013 Apr;31(2):186-90.
To evaluate the effects of non-Saccharomyces albicans metabolic products on the cell cycle distribution and proliferation of human umbilical vein endothelial cell ECV304 cells in vitro.
The parallel dilution supernatant of Saccharomyces tropicalis, Saccharomyces krusei and Saccharomyces glabrata were prepared, and 1, 4, 16-fold(s) diluted concentration and control group were set up. The line of human umbilical vein endothelial cell ECV304 was cultured in vitro and treated by non-Saccharomyces albicans supernatant. The proliferous effect of ECV304 induced by non-Saccharomyces albicans supernatant after 24, 48, 72 h was detected by the methods of MTT, and the changes of cell density and cycle after 48 h were investigated by inverted microscope and flow cytometry.
At the 24th hour, all of the higher concentration (1-fold) of non-Saccharomyces albicans supernatant and the 4-folds diluted Saccharomyces krusei could promote ECV304 proliferation(P < 0.05). After adding various non-Saccharomyces albicans supernatant at 48h and 72th hour, Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant significantly increased proliferation rate of ECV304, while Saccharomyces tropicalis supernatant group showed no significant change no matter which concentration was tested. At 48th hour after adding the non-Saccharomyces albicans supernatant, the ECV304 cells density treated by Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant were significantly higher under the inverted microscope. The G0/G1 population of ECV304 cells decreased while cell proliferation index (PI) increased after incubated with Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant for 48 hours (P < 0.05). Saccharomyces tropicalis group showed no significant change (P > 0.05).
The metabolic products of Sacharoymces krusei and Saccharomyces glabrata could induce proliferation of ECV304 cell, which suggests non-Saccharomyces albicans should be undergone more attention clinically in detection and treatment.
评估非白色念珠菌代谢产物对人脐静脉内皮细胞ECV304细胞周期分布及体外增殖的影响。
制备热带假丝酵母菌、克鲁斯假丝酵母菌和光滑假丝酵母菌的平行稀释上清液，设置1、4、16倍稀释浓度及对照组。体外培养人脐静脉内皮细胞ECV304株，用非白色念珠菌上清液处理。采用MTT法检测非白色念珠菌上清液作用24、48、72 h后对ECV304的增殖作用，通过倒置显微镜和流式细胞术观察48 h后细胞密度及周期变化。
在24 h时，所有较高浓度（1倍）的非白色念珠菌上清液以及4倍稀释的克鲁斯假丝酵母菌上清液均可促进ECV304增殖（P<0.05）。在48 h和72 h加入各种非白色念珠菌上清液后，克鲁斯假丝酵母菌上清液和光滑假丝酵母菌上清液显著提高了ECV304的增殖率，而热带假丝酵母菌上清液组无论检测哪种浓度均无显著变化。加入非白色念珠菌上清液48 h后，倒置显微镜下可见克鲁斯假丝酵母菌上清液和光滑假丝酵母菌上清液处理的ECV304细胞密度显著更高。与克鲁斯假丝酵母菌上清液和光滑假丝酵母菌上清液孵育48小时后，ECV304细胞的G0/G1期细胞减少而细胞增殖指数（PI）增加（P<0.05）。热带假丝酵母菌组无显著变化（P>0.05）。
克鲁斯假丝酵母菌和光滑假丝酵母菌的代谢产物可诱导ECV304细胞增殖，提示非白色念珠菌在临床检测和治疗中应受到更多关注。
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