反向遗传学鉴定开放阅读框 4 的产物是 Nyamanini 病毒的必需粒子组装因子。
Reverse genetics identifies the product of open reading frame 4 as an essential particle assembly factor of Nyamanini virus.
机构信息
Department of Virology, University of Freiburg, Freiburg, Germany.
出版信息
J Virol. 2013 Jul;87(14):8257-60. doi: 10.1128/JVI.00163-13. Epub 2013 May 22.
Abstract
We established a reverse genetics system for Nyamanini virus (NYMV) and recovered green fluorescent protein (GFP)-expressing virus from full-length cDNA. Using this technology, we assessed the functions of two poorly characterized viral genes. NYMV lacking open reading frame 2 (ORF2) could not be rescued, whereas virus lacking ORF4 was replication competent. ORF4-deficient NYMV readily established a persisting noncytolytic infection but failed to produce infectious viral particles, supporting the view that ORF4 represents an essential factor for NYMV particle assembly.
摘要
我们建立了尼亚马尼病毒（NYMV）的反向遗传学系统，并从全长 cDNA 中恢复了表达绿色荧光蛋白（GFP）的病毒。利用这项技术，我们评估了两个特征尚不明确的病毒基因的功能。缺失开放阅读框 2（ORF2）的 NYMV 无法被拯救，而缺失 ORF4 的病毒则具有复制能力。缺乏 ORF4 的 NYMV 容易建立持续的非细胞毒性感染，但无法产生感染性病毒颗粒，这支持了 ORF4 是 NYMV 粒子组装所必需的因素这一观点。
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