Medical Research Center Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
PLoS One. 2013 May 23;8(5):e63516. doi: 10.1371/journal.pone.0063516. Print 2013.
It is currently unclear whether the expression of HOX transcript antisense RNA (HOTAIR) correlates with the progression of esophageal cancer. The aim of this study was to examine HOTAIR expression in patients with esophageal squamous cell cancer (ESCC) and explore its clinical significance.
Differences in the expression of HOTAIR were examined via in situ hybridization (ISH) and quantitative reverse transcriptase PCR (qRT-PCR). The prognostic significance was evaluated using Kaplan-Meier and Cox regression analyses. Proliferation, colony formation and migration assays were performed in ESCC cell lines to determine the function of HOTAIR in the progression of ESCC in vitro.
A notably higher level of HOTAIR expression was found in ESCC tissues. High expression levels of HOTAIR in ESCC patients correlated positively with clinical stage, TNM classification, histological differentiation and vital status. HOTAIR expression was found to be an independent prognostic factor in ESCC patients. ESCC patients who expressed high levels of HOTAIR had substantially lower overall 5-year survival rates than HOTAIR-negative patients. In vitro assays of ESCC cell lines demonstrated that HOTAIR mediated the proliferation, colony formation and migratory capacity of ESCC cells.
HOTAIR is a potential biomarker for ESCC prognosis, and the dysregulation of HOTAIR may play an important role in ESCC progression.
HOX 转录反义 RNA（HOTAIR）的表达是否与食管癌的进展相关目前尚不清楚。本研究旨在检测食管鳞状细胞癌（ESCC）患者中 HOTAIR 的表达情况，并探讨其临床意义。
采用原位杂交（ISH）和实时定量逆转录 PCR（qRT-PCR）检测 HOTAIR 的表达差异。采用 Kaplan-Meier 和 Cox 回归分析评估其预后意义。在 ESCC 细胞系中进行增殖、集落形成和迁移实验，以确定 HOTAIR 在 ESCC 体外进展中的作用。
ESCC 组织中 HOTAIR 的表达水平明显升高。ESCC 患者中 HOTAIR 的高表达水平与临床分期、TNM 分类、组织学分化和生存状态呈正相关。HOTAIR 表达是 ESCC 患者的独立预后因素。表达高水平 HOTAIR 的 ESCC 患者的总 5 年生存率明显低于 HOTAIR 阴性患者。ESCC 细胞系的体外实验表明，HOTAIR 介导了 ESCC 细胞的增殖、集落形成和迁移能力。
HOTAIR 是 ESCC 预后的潜在生物标志物，HOTAIR 的失调可能在 ESCC 的进展中发挥重要作用。
