Han Bao-Sheng, Gao Jun-Ling
Department of Reproduction and Genetics, Maternity and Child Health Care Hospital, Tangshan, Hebei 063000, P.R. China.
Exp Ther Med. 2013 May;5(5):1281-1288. doi: 10.3892/etm.2013.1018. Epub 2013 Mar 19.
The aim of this study was to identify an optimal method for the parthenogenetic activation of mouse oocytes. Ethanol (EH), strontium chloride (SrCl) and ionomycin calcium salt were each combined with cytochalasin B to induce the parthenogenetic activation of CD-1 mouse oocytes. Among the EH combination groups, the blastocyst formation and hatching rates of the group that was activated with EH and CB for 5 min were significantly higher compared with those of the groups that were activated for 7 and 10 min (P<0.05). Among the SrCl combination groups, the blastocyst formation and hatching rates of the group that was activated with SrCl and CB for 30 min were significantly higher compared with those of the groups that were activated for 1 and 2 h (P<0.05). Among the ionomycin calcium salt combination groups, the blastocyst formation and hatching rates of the group that was activated with ionomycin and CB for 3 min were higher compared with those of the groups that were activated for 5 and 7 min (P<0.05). Compared with the other two combinations, the experimental indicators of the EH combination groups were notably superior (P<0.05). For combined activation, simultaneous activation with two substances was significantly more effective than successive activation (P<0.05). For combined activation with EH and cytochalasin B in mouse oocytes, 5 min of parthenogenetic activation had significant advantages with regard to cleavage, blastocyst formation and blastocyst hatching rates. In addition, the activation rate of combined activation was higher than that of single activators. For combined activation, the simultaneous application of two activators has a superior effect.
本研究的目的是确定一种小鼠卵母细胞孤雌激活的最佳方法。将乙醇（EH）、氯化锶（SrCl）和离子霉素钙盐分别与细胞松弛素B联合使用，以诱导CD-1小鼠卵母细胞的孤雌激活。在EH联合组中，与激活7分钟和10分钟的组相比，用EH和CB激活5分钟的组的囊胚形成率和孵化率显著更高（P<0.05）。在SrCl联合组中，与激活1小时和2小时的组相比，用SrCl和CB激活30分钟的组的囊胚形成率和孵化率显著更高（P<0.05）。在离子霉素钙盐联合组中，与激活5分钟和7分钟的组相比，用离子霉素和CB激活3分钟的组的囊胚形成率和孵化率更高（P<0.05）。与其他两种联合方法相比，EH联合组的实验指标明显更优（P<0.05）。对于联合激活，两种物质同时激活比相继激活显著更有效（P<0.05）。对于小鼠卵母细胞中EH和细胞松弛素B的联合激活，5分钟的孤雌激活在卵裂、囊胚形成和囊胚孵化率方面具有显著优势。此外，联合激活的激活率高于单一激活剂。对于联合激活，同时应用两种激活剂效果更佳。
