一种用于测定HIV感染患者血浆中犬尿氨酸和色氨酸浓度的简单液相色谱-串联质谱法。
A simple LC-MS/MS method for determination of kynurenine and tryptophan concentrations in human plasma from HIV-infected patients.
作者信息
Huang Yong, Louie Alexander, Yang Qiyun, Massenkoff Nicholas, Xu Connie, Hunt Peter W, Gee Winnie
机构信息
Drug Studies Unit, Department of Bioengineering & Therapeutic Sciences, University of California, San Francisco, CA 94143, USA.
出版信息
Bioanalysis. 2013 Jun;5(11):1397-407. doi: 10.4155/bio.13.74.
Abstract
BACKGROUND
Indoleamine 2,3-dioxygenase, catalyzing tryptophan (Trp) metabolism through the kynurenine (Kyn) metabolic pathway, plays important roles in immune suppression and the CNS. In this article, we report a simple, rapid and specific LC-MS/MS method for accurate determination of Kyn and Trp concentrations in human plasma from HIV-infected patients.
RESULTS
The human plasma sample (100 µl) was mixed with Kyn-d4 and Trp-d5 internal standards and then precipitated with trifluoroacetic acid. The supernatant was directly analyzed by LC-MS/MS. The assay using surrogate matrix calibrators was validated for precision, accuracy, matrix effect, extraction efficiency and stability. Some assay validation issues for endogenous substance bioanalysis using an LC-MS/MS method are discussed.
CONCLUSION
A simple, specific and reproducible LC-MS/MS method has been developed and validated for measuring Kyn and Trp in human plasma samples.
摘要
背景
吲哚胺2,3-双加氧酶通过犬尿氨酸代谢途径催化色氨酸（Trp）代谢，在免疫抑制和中枢神经系统中发挥重要作用。在本文中，我们报告了一种简单、快速且特异的液相色谱-串联质谱法，用于准确测定HIV感染患者血浆中犬尿氨酸（Kyn）和色氨酸（Trp）的浓度。
结果
将人血浆样品（100 μl）与Kyn-d4和Trp-d5内标混合，然后用三氟乙酸沉淀。上清液直接用液相色谱-串联质谱法分析。使用替代基质校准物的测定方法在精密度、准确度、基质效应、提取效率和稳定性方面得到了验证。讨论了使用液相色谱-串联质谱法进行内源性物质生物分析的一些测定验证问题。
结论
已开发并验证了一种简单、特异且可重复的液相色谱-串联质谱法，用于测定人血浆样品中的犬尿氨酸（Kyn）和色氨酸（Trp）。
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