Department of Environmental Science, Graduate School of Environmental Studies, Tohoku University, Aoba-ku, Sendai 9808579, Japan.
Bioresour Technol. 2013 Aug;142:304-11. doi: 10.1016/j.biortech.2013.04.102. Epub 2013 May 13.
Three continuous stirred tank reactors (CSTR) were operated under mesophilic (37 ± 1°C), thermophilic (55 ± 1°C) and hyper-thermophilic (80 ± 1°C) temperatures for 164 days to investigate the effect of temperature and temperature shock on the cellulosic-dark hydrogen fermentation by mixed microflora. During steady state condition, the sudden decreases in the fermentation temperature occurred twice in each condition for 24h. The results show that the 55 ± 1 and 80 ± 1°C presented stable hydrogen yields of 12.28 and 9.72 mmol/g cellulose, respectively. However, the 37 ± 1°C presented low hydrogen yield of 3.56 mmol/g cellulose and methane yield of 5.4 mmol/g cellulose. The reactor performance under 55 ± 1 or 80 ± 1°C appeared to be more resilient to the sudden decreases in the fermentation temperature than 37 ± 1°C. The experimental analysis results indicated that the changing in soluble by-products could explain the effect of temperature and temperature shock, and the thermophilic temperature is expected having a better economic performance for cellulosic-hydrogen fermentation.
三个连续搅拌釜式反应器（CSTR）在中温（37±1°C）、高温（55±1°C）和超高温（80±1°C）下运行 164 天，以研究温度和温度冲击对混合微生物群进行纤维素-暗氢发酵的影响。在稳定状态下，每种条件下发酵温度突然下降两次，持续 24 小时。结果表明，55±1°C 和 80±1°C 分别呈现出稳定的 12.28mmol/g 纤维素和 9.72mmol/g 纤维素的产氢量。然而，37±1°C 的产氢量较低，为 3.56mmol/g 纤维素，产甲烷量为 5.4mmol/g 纤维素。55±1°C 或 80±1°C 下的反应器性能似乎比 37±1°C 对发酵温度的突然下降更具弹性。实验分析结果表明，可溶性副产物的变化可以解释温度和温度冲击的影响，预计高温对纤维素-氢气发酵具有更好的经济性能。
