淹水胁迫下大豆子叶和根的蛋白质组学和生化分析。
Proteomic and biochemical analyses of the cotyledon and root of flooding-stressed soybean plants.
机构信息
National Institute of Crop Science, NARO, Tsukuba, Japan.
出版信息
PLoS One. 2013 Jun 14;8(6):e65301. doi: 10.1371/journal.pone.0065301. Print 2013.
BACKGROUND
Flooding significantly reduces the growth and grain yield of soybean plants. Proteomic and biochemical techniques were used to determine whether the function of cotyledon and root is altered in soybean under flooding stress.
RESULTS
Two-day-old soybean plants were flooded for 2 days, after which the proteins from root and cotyledon were extracted for proteomic analysis. In response to flooding stress, the abundance of 73 and 28 proteins was significantly altered in the root and cotyledon, respectively. The accumulation of only one protein, 70 kDa heat shock protein (HSP70) (Glyma17g08020.1), increased in both organs following flooding. The ratio of protein abundance of HSP70 and biophoton emission in the cotyledon was higher than those detected in the root under flooding stress. Computed tomography and elemental analyses revealed that flooding stress decreases the number of calcium oxalate crystal the cotyledon, indicating calcium ion was elevated in the cotyledon under flooding stress.
CONCLUSION
These results suggest that calcium might play one role through HSP70 in the cotyledon under flooding stress.
背景
洪水会显著降低大豆植株的生长和籽粒产量。本研究采用蛋白质组学和生物化学技术，以确定在水淹胁迫下大豆子叶和根的功能是否发生改变。
结果
将 2 日龄的大豆植株水淹 2 天，之后提取根和子叶的蛋白质进行蛋白质组学分析。响应水淹胁迫，根和子叶中分别有 73 种和 28 种蛋白质的丰度显著改变。仅有一种蛋白质，即 70 kDa 热休克蛋白（HSP70）（Glyma17g08020.1），在两个器官中均有积累，在水淹后增加。子叶中 HSP70 与生物发光比值高于根中检测到的比值。计算机断层扫描和元素分析表明，水淹胁迫会减少子叶中的草酸钙晶体数量，表明在水淹胁迫下子叶中的钙离子水平升高。
结论
这些结果表明，在水淹胁迫下，钙可能通过 HSP70 发挥作用。
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