男性前列腺分泌物在人乳头瘤病毒流行病学研究中的表达。
Expressed prostate secretions in the study of human papillomavirus epidemiology in the male.
机构信息
Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
出版信息
PLoS One. 2013 Jun 14;8(6):e66630. doi: 10.1371/journal.pone.0066630. Print 2013.
INTRODUCTION
Exploring different sampling sites and methods is of interest for studies of the epidemiology of HPV infections in the male. Expressed prostate secretions (EPS) are obtained during digital rectal examination (DRE), a daily routine urological diagnostic procedure, following massage of the prostate.
MATERIALS AND METHODS
Urethral swabs and EPS samples were obtained from a consecutive sample of 752 men (mean age 32.4 years; median life-time sex partners 34) visiting urology outpatient clinics in St. Petersburg, Russia and tested for HPV DNA by general primer PCR, followed by genotyping using Luminex.
RESULTS
Overall, 47.9% (360/752) of men were HPV-positive, with 42.0% (316/752) being positive for high-risk (HR-) HPV and 12.6% (95/752) for multiple HPV types. HPV-positivity in the EPS samples was 32.6% (27.7% HR-HPV) and in the urethral samples 25.9% (24.5% HR-HPV). 10.6% were HPV positive in both EPS and urethral samples. 6.4% had the same HPV-type in both EPS and urethral samples. 10.6% were HPV positive in both EPS and urethral samples. 6.4% had the same HPV-type in both EPS and urethral samples. The concordance between the urethral samples and EPS was 62.5% (470/752), with 80 cases double positive and 390 cases double negative in both sites. The sensitivity of urethral samples for overall HPV detection was 54.2% (195/360). Compared to analysis of urethral samples only, the analysis of EPS increased the HPV prevalence in this population with 26.2%.
CONCLUSION
EPS represent informative sampling material for the study of HPV epidemiology in the male.
简介
研究男性 HPV 感染的流行病学，不同的采样部位和方法很有意义。经直肠前列腺按摩（DRE）获得的前列腺分泌物（EPS）可在泌尿科门诊中获得，这是一种日常的泌尿科诊断程序。
材料和方法
我们从俄罗斯圣彼得堡的泌尿科门诊连续采集了 752 名男性的尿道拭子和 EPS 样本（平均年龄 32.4 岁；中位数终生性伴侣数 34），通过通用引物 PCR 检测 HPV DNA，然后使用 Luminex 进行基因分型。
结果
752 名男性中，总体 HPV 阳性率为 47.9%（360/752），其中高危型（HR-）HPV 阳性率为 42.0%（316/752），多重 HPV 型阳性率为 12.6%（95/752）。EPS 样本 HPV 阳性率为 32.6%（27.7%HR-HPV），尿道样本 HPV 阳性率为 25.9%（24.5%HR-HPV）。10.6%的男性 EPS 和尿道样本均为 HPV 阳性。6.4%的男性 EPS 和尿道样本的 HPV 型相同。10.6%的男性 EPS 和尿道样本均为 HPV 阳性。6.4%的男性 EPS 和尿道样本的 HPV 型相同。EPS 和尿道样本之间的一致性为 62.5%（470/752），80 例同时为双阳性，80 例同时为双阴性。尿道样本对总体 HPV 检测的敏感性为 54.2%（195/360）。与仅分析尿道样本相比，分析 EPS 可使该人群 HPV 检出率增加 26.2%。
结论
EPS 是研究男性 HPV 流行病学的有价值的采样材料。
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