Geffner M E, Bersch N, Lippe B M, Rosenfeld R G, Hintz R L, Golde D W
Department of Pediatrics, University of California, Los Angeles Medical Center 90024.
J Clin Endocrinol Metab. 1990 Aug;71(2):464-9. doi: 10.1210/jcem-71-2-464.
It has become evident that locally produced insulin-like growth factors-I and -II (IGF-I and IGF-II) play an important role in the medication of GH action upon tissues. To explore this concept with respect to immunocompetent cells, we analyzed IGF production and clonogenic responsiveness of immortalized human T-cell lines established from seven normal controls and four Laron dwarfs. While the normal T-cell lines showed significant augmentation of basal colony formation in response to both IGF-I and GH, little increase in clonogenesis in response to GH was seen with the Laron T-cell lines. Assay of basal and GH-stimulated conditioned media demonstrated low, but measurable, levels of IGF-I and IGF-II from both normal and Laron T-cells. Under serum-free incubation conditions, GH stimulation of normal T-cell lines failed to generate significant increases in mean IGF-I or IGF-II concentration and no increase in the mean IGF-II concentration in conditioned medium were observed after GH stimulation of Laron T-cell lines. Nevertheless, the increased cloning efficiency of the normal T-cell lines in response to either GH or IGF-I was nearly completely abrogated by preincubation of cells with antibodies to either IGF-I or the type I IGF receptor. These studies, thus, support a role for locally generated IGF-I in the mediation of GH action on T-lymphocytes and indicate that this effect is mediated via the type I IGF receptor.
越来越明显的是，局部产生的胰岛素样生长因子-I和-II（IGF-I和IGF-II）在生长激素对组织的作用调节中发挥重要作用。为了在免疫活性细胞方面探讨这一概念，我们分析了从7名正常对照者和4名拉伦侏儒症患者建立的永生化人T细胞系的IGF产生情况和克隆形成反应性。正常T细胞系对IGF-I和生长激素均显示基础集落形成显著增加，而拉伦T细胞系对生长激素的克隆形成反应几乎没有增加。对基础和生长激素刺激的条件培养基的检测表明，正常和拉伦T细胞均有低水平但可测量的IGF-I和IGF-II。在无血清培养条件下，生长激素刺激正常T细胞系未能使平均IGF-I或IGF-II浓度显著增加，拉伦T细胞系经生长激素刺激后，条件培养基中的平均IGF-II浓度也未增加。然而，用抗IGF-I或I型IGF受体抗体预孵育细胞后，正常T细胞系对生长激素或IGF-I的克隆效率增加几乎完全被消除。因此，这些研究支持局部产生的IGF-I在生长激素对T淋巴细胞作用的调节中发挥作用，并表明这种作用是通过I型IGF受体介导的。
