University Leipzig-Heart Center Leipzig, Department of Cardiology, Leipzig, Germany; Takasaki General Medical Center, Division of Cardiology, Takasaki, Japan; Gunma University School of Medicine, Department of Cardiology, Maebashi, Japan.
Obesity (Silver Spring). 2013 Dec;21(12):E687-95. doi: 10.1002/oby.20538. Epub 2013 Aug 13.
HDL regulates endothelial function via stimulation of nitric oxide production. It is documented that endothelial function is impaired in obese adolescents, and improved by lifestyle interventions (LI).
HDL function in obese adolescents and the impact of LI or Roux-en-Y gastric bypass surgery (RYGB) was assessed. HDL was isolated from 14 adolescents with normal body mass index (HDLcontrol ), 10 obese (HDLobese ) before and after 6 month LI, and five severe obese adolescents before and one year after RYGB. HDL-mediated phosphorylation of endothelial nitric oxide synthase (eNOS)-Ser(1177) , eNOS-Thr(495) , and PKC-ßII was evaluated. In addition the HDL proteome was analyzed.
HDLobese -mediated eNOS-Ser(1177) phosphorylation was reduced, whereas eNOS-Thr(495) phosphorylation increased significantly when compared to HDLcontrol . No impact of obesity was observed on PKC-ßII phosphorylation. LI and RYGB had no impact on HDL-mediated phosphorylation of eNOS and PKC-ßII. A principle component plot analysis of the HDL particle separated controls and severe obese, whereas the interventions did not trigger sufficient differences to the HDL proteome to permit distinction.
These results demonstrated that HDL-function is impaired in obese adolescents, and that LI or RYGB did not correct this dysfunction. This might be an argument for developing earlier prevention strategies in obese adolescents to avoid HDL dysfunction.
HDL 通过刺激一氧化氮的产生来调节内皮功能。有文献记录，肥胖青少年的内皮功能受损，生活方式干预（LI）可以改善这种情况。
评估了肥胖青少年的 HDL 功能以及 LI 或 Roux-en-Y 胃旁路手术（RYGB）的影响。从 14 名体重指数正常的青少年（HDLcontrol）、10 名肥胖青少年（HDLobese）的 HDL 中分离出来，在接受 6 个月 LI 前后，以及 5 名严重肥胖青少年的 HDL 中分离出来，在接受 RYGB 前和一年后。评估了 HDL 介导的内皮型一氧化氮合酶（eNOS）-Ser（1177）、eNOS-Thr（495）和 PKC-ßII 的磷酸化。此外，还分析了 HDL 的蛋白质组。
与 HDLcontrol 相比，HDLobese 介导的 eNOS-Ser（1177）磷酸化减少，而 eNOS-Thr（495）磷酸化显著增加。肥胖对 PKC-ßII 磷酸化没有影响。LI 和 RYGB 对 HDL 介导的 eNOS 和 PKC-ßII 磷酸化没有影响。HDL 颗粒分离的对照和严重肥胖的主成分图分析，而干预措施并没有引起足够的差异，使 HDL 蛋白质组能够区分。
这些结果表明，肥胖青少年的 HDL 功能受损，LI 或 RYGB 并不能纠正这种功能障碍。这可能是在肥胖青少年中尽早采取预防策略以避免 HDL 功能障碍的一个论据。
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