Rakovskaia I V, Gorina L G, Balabanov D N, Levina G A, Barkhatova O I, Goncharova S A, Gamova N A
Zh Mikrobiol Epidemiol Immunobiol. 2013 Mar-Apr(2):37-43.
Study of possibility of generalization of mycoplasma infection in patients with urogenital pathology.
Among the examined patients 5 males characterized by risky sexual behavior with pronounced symptoms of infection or without those were selected. Patients were examined by a complex of methods for the presence of mycoplasma infection by culture, PCR, DFA, PHA, AHR and by detection of specific immune complexes in blood sera. Scrapes from urogenital tract, blood sera samples, urine, saliva, prostatic fluid were materials for the study.
In blood of all patients in ELISA antibodies against Mycoplasma hominis were detected; in PHA they were detected only in 2 individuals. In all the patients in blood CIC were detected including antigens and DNA of one or several mycoplasma species. Sperm of 3 individuals was infected by Ureaplasma spp., 2--M. genitalium. In saliva of 2 individuals M. hominis was detected, 3--U. urealyticum.
In all the examined patients the infection was shown to have generalized character. This phenomenon presents itself as quite significant because mycoplasma may cause anti-apoptotic and oncogenic effect.
研究泌尿生殖系统疾病患者支原体感染的播散可能性。
在接受检查的患者中,选择5名有高危性行为且有明显感染症状或无明显症状的男性。通过培养、聚合酶链反应(PCR)、直接荧光抗体法(DFA)、血凝试验(PHA)、吸附血凝试验(AHR)等一系列方法检测患者是否存在支原体感染,并检测血清中的特异性免疫复合物。取自泌尿生殖道的刮片、血清样本、尿液、唾液、前列腺液均作为研究材料。
所有患者的酶联免疫吸附测定(ELISA)血液中均检测到抗人型支原体抗体;血凝试验中仅在2名个体中检测到。所有患者血液中均检测到循环免疫复合物(CIC),包括一种或几种支原体的抗原和DNA。3名个体的精液感染了解脲脲原体,2名个体感染了生殖支原体。在2名个体的唾液中检测到人型支原体,3名个体检测到解脲脲原体。
所有接受检查的患者均显示感染具有播散性。这种现象相当显著,因为支原体可能会产生抗凋亡和致癌作用。