[循环免疫复合物作为支原体细胞成分的保守储存库]
[Circulating immune complexes as a depot of conservation of mycoplasma cell components].
作者信息
Gorina L G, Rakovskaia I V, Barkhatova O I, Goncharova S A, Levina G A, Krylova N A
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 2013 Mar-Apr(2):74-82.
AIM
Study the possibility of prolonged conservation in macroorganism of antigens, mycoplasma cell DNA and live pathogen cells as part of CIC against the background of persisting antigen biostructures.
MATERIALS AND METHODS
Aggregate-hemagglutination, direct immunofluorescence reactions and PCR method were used to determine antigens and DNA. Circulating immune complexes from blood sera samples were isolated by M. Digeon et al., mycoplasma isolation from CIC was carried out in SP-4 medium, species identity of the isolated mini-colonies was confirmed by real-time PCR method.
RESULTS
In patients with urogenital and respiratory pathology the frequency of detection of Mycoplasma hominis, Ureaplasma urealyticum and Mycoplasma pneumoniae in free state was 63.3, 53.1 and 80.82% of cases, respectively. Specific CIC in patients with verified respiratory mycoplasmosis 1 month after the onset of the disease were registered in patients with severe course of the disease, bronchitis and diseases of upper respiratory tract--in 92.5, 74.7 and 25.7% of cases, respectively. In children, bronchial asthma patients the frequency of detection of antigens and DNA of M. pneumoniae cells in free state was 72.6 and 12.33%, as part of CIC--in 60.27 and 43.8% of cases, respectively. Antigens and DNA of M. hominis in blood of this group of patients were detected in 32.9 and 26.02%, as part of CIC--in 53.42 and 52.05% of cases, respectively. During repeated examination of 12 children after etiotropic therapy execution (generally in 1.5 - 6 months) in 75% of cases antigens of both M. pneumoniae and M. hominis were detected in free state and as part of CIC. DNA of cells of these mycoplasma species were detected in 20 and 33%, as part of CIC--in41.6 and 50% of cases, respectively. In 5 patients after 6 months (after 1 year in 1 case) mycoplasma antigens and DNA were identified in CIC or in blood sera. During cultivation of CIC components precipitated from 5 blood samples of patients of this group containing M. hominis DNA, culture of M. hominis mini-colonies were isolated in 4 cases.
CONCLUSION
The possibility of prolonged persistence of antigens, DNA and whole mycoplasma cells in both free state and as part of CIC in patients with respiratory and urogenital pathology was shown. CIC are thus a peculiar depot, a place of conservation of not only various mycoplasma cell components, but also live cells.
目的
研究在持续存在抗原生物结构的背景下，作为循环免疫复合物（CIC）一部分的抗原、支原体细胞DNA和活病原体细胞在大生物体中长时间保存的可能性。
材料与方法
采用凝集-血凝反应、直接免疫荧光反应和聚合酶链反应（PCR）方法来测定抗原和DNA。采用M. Digeon等人的方法从血清样本中分离循环免疫复合物，在SP-4培养基中从CIC中分离支原体，通过实时PCR方法确认分离出的小菌落的种属同一性。
结果
在泌尿生殖系统和呼吸道疾病患者中，人型支原体、解脲脲原体和肺炎支原体游离状态的检出率分别为病例的63.3%、53.1%和80.82%。经确诊的呼吸道支原体病患者在发病1个月后，重症患者、支气管炎患者和上呼吸道疾病患者中特异性CIC的检出率分别为92.5%、74.7%和25.7%。在儿童支气管哮喘患者中，肺炎支原体细胞抗原和DNA游离状态的检出率分别为72.6%和12.33%，作为CIC一部分的检出率分别为60.27%和43.8%。该组患者血液中人型支原体抗原和DNA的检出率分别为32.9%和26.02%，作为CIC一部分的检出率分别为53.42%和52.05%。在对12名儿童进行病因治疗后（一般在1.5 - 6个月）的再次检查中，75%的病例中同时检测到了游离状态和作为CIC一部分的肺炎支原体和人型支原体抗原。这些支原体物种细胞的DNA检出率分别为20%和33%，作为CIC一部分的检出率分别为41.6%和50%。在5名患者6个月后（1例为1年后），在CIC或血清中鉴定出了支原体抗原和DNA。在从该组5名含有人型支原体DNA的患者血液样本中沉淀的CIC成分培养过程中，4例分离出了人型支原体小菌落培养物。
结论
研究表明，在呼吸道和泌尿生殖系统疾病患者中，抗原、DNA和完整的支原体细胞不仅可以以游离状态，还可以作为CIC的一部分长时间存在。因此，CIC是一个特殊的储存库，不仅是各种支原体细胞成分的保存场所，也是活细胞的保存场所。
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