产气荚膜梭菌A型cpb2编码与新生仔猪腹泻之间关联的调查。
An investigation into the association between cpb2-encoding Clostridium perfringens type A and diarrhea in neonatal piglets.
作者信息
Farzan Abdolvahab, Kircanski Jasmina, DeLay Josepha, Soltes Glenn, Songer J Glenn, Friendship Robert, Prescott John F
机构信息
Department of Population Medicine, Animal Health Laboratory, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
出版信息
Can J Vet Res. 2013 Jan;77(1):45-53.
To investigate the possible role of cpb2-positive type A Clostridium perfringens in neonatal diarrheal illness in pigs, the jejunum and colon of matched normal and diarrheic piglets from 10 farms with a history of neonatal diarrhea were examined grossly and by histopathology, and tested for C. perfringens, for C. perfringens beta2 (CPB2) toxin, as well as for Clostridium difficile toxins, Salmonella, enterotoxigenic Escherichia coli, rotavirus, transmissible gastroenteritis (TGE) virus, and coccidia. Clostridium perfringens isolates were tested using a multiplex real-time polymerase chain reaction (PCR) to determine the presence of cpa, consensus and atypical cpb2, and other virulence-associated genes. The numbers of C. perfringens in the intestinal contents were lower in diarrheic piglets (log₁₀ 5.4 CFU/g) compared with normal piglets (log₁₀ 6.5 CFU/g) (P < 0.05). The consensus cpb2 was present in 93% of isolates in each group, but atypical cpb2 was less common (56% healthy, 32% diarrheic piglets isolates, respectively, P < 0.05). The presence of CPB2 toxin in the intestinal contents of normal and diarrheic piglets did not differ significantly. Clostridium difficile toxins and rotavirus were each detected in 7 of the 21 (33%) diarrheic piglets. Rotavirus, C. difficile toxins, Salmonella, or enterotoxigenic E. coli were concurrently recovered in different combinations in 4 diarrheic piglets. The cause of diarrhea in 8 of the 21 (38%) piglets on 6 farms remained unknown. The etiological diagnosis of diarrhea could not be determined in any of the piglets on 2 of the farms. This study demonstrated that the number of cpb2-positive type A C. perfringens in the intestinal contents was not a useful approach for making a diagnosis of type A C. perfringens enteritis in piglets. Further work is required to confirm whether cpb2-carrying type A C. perfringens have a pathogenic role in enteric infection in neonatal swine.
为研究产气荚膜梭菌cpb2阳性A型菌株在仔猪新生腹泻病中可能发挥的作用，对来自10个有新生腹泻病史猪场的匹配正常和腹泻仔猪的空肠和结肠进行大体检查和组织病理学检查，并检测产气荚膜梭菌、产气荚膜梭菌β2（CPB2）毒素、艰难梭菌毒素、沙门氏菌、产肠毒素大肠杆菌、轮状病毒、传染性胃肠炎（TGE）病毒和球虫。使用多重实时聚合酶链反应（PCR）检测产气荚膜梭菌分离株，以确定cpa、共有型和非典型cpb2以及其他毒力相关基因的存在情况。与正常仔猪（log₁₀ 6.5 CFU/g）相比，腹泻仔猪肠道内容物中产气荚膜梭菌数量更低（log₁₀ 5.4 CFU/g）（P < 0.05）。每组93%的分离株中存在共有型cpb2，但非典型cpb2较少见（分别为健康仔猪分离株的56%、腹泻仔猪分离株的32%，P < 0.05）。正常和腹泻仔猪肠道内容物中CPB2毒素的存在情况无显著差异。21头腹泻仔猪中有7头（33%）检测出艰难梭菌毒素和轮状病毒。4头腹泻仔猪中同时以不同组合检出轮状病毒、艰难梭菌毒素、沙门氏菌或产肠毒素大肠杆菌。6个猪场的21头仔猪中有8头（38%）腹泻病因不明。2个猪场的任何仔猪均无法确定腹泻的病因诊断。本研究表明，肠道内容物中cpb2阳性A型产气荚膜梭菌数量并非诊断仔猪A型产气荚膜梭菌肠炎的有用方法。需要进一步开展工作以确认携带cpb2的A型产气荚膜梭菌在新生仔猪肠道感染中是否具有致病作用。
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