Mangas Arturo, Vecino Elena, David Rodríguez F, Geffard Michel, Coveñas Rafael
Laboratory of Neuroanatomy of the Peptidergic Systems (Lab. 14), Salamanca, Spain.
Neurol Res. 2013 Nov;35(9):903-11. doi: 10.1179/1743132813Y.0000000233. Epub 2013 Jun 20.
Chronic experimental autoimmune encephalomyelitis (EAE) was induced in rats to evaluate the potential protective effect of GEMSP, a mixture made up of fatty acids (FA), vitamins, and amino acids or their derivatives, linked to Poly-L-Lysine, on the myelin sheath of the optic nerve.
To evaluate the effects of GEMSP on the optic nerve, animals were divided into three experimental groups: (1) EAE rats treated with GEMSP; (2) EAE rats treated with 0.9% NaCl; and (3) control, non-EAE rats. Using electron microscopy, we investigated the possibility that this new drug candidate has a myelin-protective role.
A marginally significant reduction in the thickness of the myelin around optic nerve medium-size axons (diameter between 0.8-1.3 μm) was found in EAE rats. Treatment of EAE rats with GEMSP ameliorated myelin damage. Significantly increased myelin thickness was found when animals in groups 2 and 3 were compared. However, the number of myelinated axons studied was not altered in groups 1 or 2 when compared to controls.
Our results suggest that in a model of demyelination, GEMSP protects and enhances the formation of the myelin sheath of the optic nerve and therefore could be a potential drug candidate to reduce optic nerve pathogenesis in multiple sclerosis (MS).
在大鼠中诱导慢性实验性自身免疫性脑脊髓炎（EAE），以评估由脂肪酸（FA）、维生素和氨基酸或其衍生物与聚-L-赖氨酸连接而成的混合物GEMSP对视神经髓鞘的潜在保护作用。
为了评估GEMSP对视神经的影响，将动物分为三个实验组：（1）用GEMSP治疗的EAE大鼠；（2）用0.9%氯化钠治疗的EAE大鼠；（3）对照非EAE大鼠。我们使用电子显微镜研究了这种新的候选药物具有髓鞘保护作用的可能性。
在EAE大鼠中，发现视神经中等大小轴突（直径在0.8 - 1.3μm之间）周围的髓鞘厚度略有显著降低。用GEMSP治疗EAE大鼠可改善髓鞘损伤。当比较第2组和第3组动物时，发现髓鞘厚度显著增加。然而，与对照组相比，第1组或第2组中研究的有髓轴突数量没有改变。
我们的结果表明，在脱髓鞘模型中，GEMSP保护并增强视神经髓鞘的形成，因此可能是一种潜在的药物，可减少多发性硬化症（MS）中的视神经病变。
Zotero 插件