Brodersen Ditlev Egeskov, Andersen Gregers Rom, Andersen Christian Brix Folsted
Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10c, DK-8000 Aarhus C, Denmark.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul;69(Pt 7):815-20. doi: 10.1107/S1744309113014425. Epub 2013 Jun 29.
In this paper, a simple low-cost alternative to large commercial systems for preparing macromolecular crystallization conditions is described. Using an intuitive spreadsheet-based approach, the system allows the rapid calculation of relevant pipetting volumes given known stock-solution concentrations and incorporates the automatic design of custom crystallization screens via the incomplete-factorial and grid-screen approaches. Automated dispensing of the resulting crystallization screens is achieved using a generic and relatively inexpensive liquid handler.
本文描述了一种用于制备大分子结晶条件的简单低成本替代大型商业系统的方法。该系统采用基于直观电子表格的方法，在已知储备溶液浓度的情况下，能够快速计算相关移液体积，并通过不完全因子法和网格筛选法自动设计定制的结晶筛选方案。使用通用且相对便宜的液体处理仪可实现对所得结晶筛选方案的自动分配。
