从牛脑中分离谷氨酰牛磺酸并通过B/E链接扫描二次离子质谱技术证明其γ-连接。

Isolation of glutamyltaurine from bovine brains and proof of its gamma-linkage by the B/E linked scan SIMS technique.

作者信息

Nakamura K, Higashiura K, Nishimura N, Yamamoto A, Tooyama I, Kimura H, Ienaga K

机构信息

Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Co., Ltd., Hyogo, Japan.

出版信息

J Neurochem. 1990 Sep;55(3):1064-6. doi: 10.1111/j.1471-4159.1990.tb04598.x.

DOI:10.1111/j.1471-4159.1990.tb04598.x

PMID:2384748

Abstract

We isolated a glutamyltaurine from bovine brains and determined its structure as gamma-glutamyltaurine (gamma-Glu-Tau; glutaurine) by use of a new mass spectrometric technique [B/E linked scan sputtered ion mass spectrometry (SIMS)], which we have recently shown to be useful for distinguishing the gamma- from the alpha-isomer of glutamyl-dipeptides. Neither the alpha-isomer of glutamyltaurine nor any aspartyltaurines could be detected in bovine brain.

摘要

我们从牛脑中分离出一种谷氨酰牛磺酸，并通过一种新的质谱技术[B/E 链接扫描溅射离子质谱法（SIMS）]确定其结构为γ-谷氨酰牛磺酸（γ-Glu-Tau；谷氨酰胺），我们最近证明该技术可用于区分谷氨酰二肽的γ-异构体和α-异构体。在牛脑中未检测到谷氨酰牛磺酸的α-异构体或任何天冬氨酰牛磺酸。