具有广泛底物特异性的人工 RNA 连接酶对 5'-三磷酸 RNA 的通用标记。
Universal labeling of 5'-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity.
机构信息
Department of Biochemistry, Molecular Biology and Biophysics & BioTechnology Institute, University of Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA.
出版信息
Chem Commun (Camb). 2013 Aug 25;49(66):7322-4. doi: 10.1039/c3cc44454f.
Abstract
An artificial RNA ligase specific to RNA with a 5'-triphosphate (PPP-RNA) exhibits broad sequence specificity on model substrates and secondary siRNAs with direct applications in the identification of PPP-RNAs through sequencing.
摘要
一种对具有 5′-三磷酸基团（PPP-RNA）的 RNA 具有特异性的人工 RNA 连接酶，在模型底物和二级 siRNA 上表现出广泛的序列特异性，可直接应用于通过测序鉴定 PPP-RNA。
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