人源微小RNA-181a-5p在胃癌中的表达及其对细胞增殖的影响。
Hsa-miR-181a-5p expression and effects on cell proliferation in gastric cancer.
作者信息
Chen Gang, Shen Zhi-Li, Wang Ling, Lv Chun-Ye, Huang Xin-En, Zhou Rong-Ping
机构信息
Department of Oncology, the Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, China.
出版信息
Asian Pac J Cancer Prev. 2013;14(6):3871-5. doi: 10.7314/apjcp.2013.14.6.3871.
PURPOSE
MicroRNAs (miRNAs) are small endogenous, non-coding, single-stranded RNAs (approximately 22 nt). Accumulating evidence has shown that aberrant miRNA expression is pronounced and correlated with gastric cancer genesis and progression.
MATERIALS AND METHODS
Expression levels of miR-181a-5p in GC tissues and cell lines were assessed by qRT-PCR and tested for correlation with clinical features. In addition, effects of miR-181a-5p on GC cell growth were investigated.
RESULTS
Our findings indicate that miR-181a-5p is upregulated in GC, in correlation with lymph node invasion, nerve invasion and vascular invasion (P<0.05). Enforced expression of miR-181a -5p promoted cell proliferation ability.
CONCLUSIONS
This study suggested that increased miR-181a-5p is related to GC progression. MiR-181a-5p may represent a potential therapeutic target for GC.
目的
微小RNA（miRNA）是内源性、非编码、单链小RNA（约22个核苷酸）。越来越多的证据表明，miRNA表达异常明显，且与胃癌的发生和进展相关。
材料与方法
采用qRT-PCR评估miR-181a-5p在胃癌组织和细胞系中的表达水平，并检测其与临床特征的相关性。此外，研究了miR-181a-5p对胃癌细胞生长的影响。
结果
我们的研究结果表明，miR-181a-5p在胃癌中上调，与淋巴结浸润、神经浸润和血管浸润相关（P<0.05）。miR-181a -5p的过表达促进了细胞增殖能力。
结论
本研究提示miR-181a-5p表达增加与胃癌进展相关。miR-181a-5p可能是胃癌的一个潜在治疗靶点。
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