海洋动物中的彗星试验。
The comet assay in marine animals.
作者信息
Frenzilli Giada, Lyons Brett P
机构信息
Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
出版信息
Methods Mol Biol. 2013;1044:363-72. doi: 10.1007/978-1-62703-529-3_19.
PMID:23896887
Abstract
Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe the protocols for the single-cell gel electrophoresis (Comet) assay in its alkaline (pH > 13), mild alkaline (pH = 12.1), and neutral (pH = 8) versions, when applied in marine animals.
摘要
彗星试验是一种快速且通用的技术，用于评估单个细胞中的DNA损伤。它能够检测DNA单链和双链断裂，以及碱不稳定位点和交联的存在。在此，我们描述了单细胞凝胶电泳（彗星试验）在碱性（pH > 13）、轻度碱性（pH = 12.1）和中性（pH = 8）条件下应用于海洋动物时的实验方案。
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